Development of microsatellites from the rare ironstone endemic, Tetratheca paynterae ssp. paynterae and cross-species amplification

Nineteen microsatellite markers were developed from Tetratheca paynterae ssp. paynterae, a rare and endangered, leafless, perennial shrub. Twelve loci were polymorphic in T. paynterae ssp. paynterae with two to 14 alleles per locus and mean expected heterozygosity of 0.62. Primer pairs were tested o...

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Published inMolecular ecology resources Vol. 9; no. 1; pp. 386 - 389
Main Authors BUTCHER, P.A., KRAUSS, S.L.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.01.2009
Subjects
cross-species amplification
ironstone endemic
SSR
Tetratheca
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Summary:Nineteen microsatellite markers were developed from Tetratheca paynterae ssp. paynterae, a rare and endangered, leafless, perennial shrub. Twelve loci were polymorphic in T. paynterae ssp. paynterae with two to 14 alleles per locus and mean expected heterozygosity of 0.62. Primer pairs were tested on four other Tetratheca species from ironstone ranges in southern Western Australia. Ten loci were polymorphic in T. paynterae ssp. cremnobata and T. aphylla ssp. aphylla, three in T. harperi and four in T. erubescens. The level of polymorphism was adequate for studies of genetic structure and mating systems in three of the five taxa.
Bibliography:ark:/67375/WNG-V05JTK9D-7
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ArticleID:MEN2236
ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2008.02236.x