Effect of temperature on the separation of chlorophenoxy acids and carbamates by capillary high-performance liquid chromatography and UV (or diode array) detection

• Published in: Journal of Chromatography A Vol. 1081; no. 1; pp. 114 - 121
• Main Authors: Rosales-Conrado, N., León-González, M.E., Pérez-Arribas, L.V., Polo-Díez, L.M.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 15.07.2005; Elsevier
• Subjects: Analytical chemistry; carbamate pesticides; Carbamates; Carbamates - isolation & purification; Chemistry; Chlorophenoxy acids; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; detection; diode array detection; Exact sciences and technology; Herbicides - isolation & purification; organochlorine pesticides; Other chromatographic methods; Pesticides; Phenols - isolation & purification; Programmed temperature; reversed-phase capillary high performance liquid chromatography; reversed-phase high performance liquid chromatography; Spectrophotometry, Ultraviolet; Temperature; Temperature effects; ultraviolet radiation; Programmed temperature; Carbamates; Temperature effects; Chlorophenoxy acids; Pesticides; Elution; Gradient; Peak resolution; Insecticide; Chromatographic retention; HPLC chromatography; Temperature effect; Retention time; Organic carbamate; Isocratic condition; Herbicide; Operating conditions; Ultraviolet detector; Carboxylic acid; Plate efficiency
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.12.083

In this work, the effect of temperature in isothermal and programmed modes on several chromatographic parameters such as retention factor, selectivity, resolution and plate number has been discussed. A critical comparison of isocratic/isothermal, gradient/isothermal and isocratic/program temperature modes has been made. Two representative families of pesticides have been selected for this study. One includes ionisable chlorophenoxy acids and two of their esters, some of which show similar polarities. The other one contains several weakly polar carbamates. Analysis was carried out using a reversed-phase capillary high-performance liquid chromatography (HPLC) system and focusing technique with UV or diode array detection (DAD).