Electrochemiluminescence biosensor based on molybdenum disulfide-graphene quantum dots nanocomposites and DNA walker signal amplification for DNA detection

• Published in: Mikrochimica acta (1966) Vol. 188; no. 10; p. 353
• Main Authors: Sun, Yina, Huang, Chuan, Sun, Xiujin, Wang, Qian, Zhao, Peini, Ge, Shenguang, Yu, Jinghua
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.10.2021; Springer; Springer Nature B.V
• Subjects: Amplification; Analysis; Analytical Chemistry; Biosensing Techniques - methods; Biosensors; Characterization and Evaluation of Materials; Charge transfer; Chemistry; Chemistry and Materials Science; Detectors; Disulfides - chemistry; DNA; DNA - chemistry; DNA sequencing; Electrochemical Techniques - methods; Electrochemiluminescence; Exodeoxyribonucleases - chemistry; Exonucleases; Gene sequencing; Genetic research; Graphene; Graphite; Graphite - chemistry; Limit of Detection; Luminescent Measurements - methods; Microengineering; Molybdenum; Molybdenum - chemistry; Molybdenum compounds; Molybdenum disulfide; Nanochemistry; Nanocomposites; Nanocomposites - chemistry; Nanotechnology; Nucleic Acid Amplification Techniques - methods; Nucleotide sequencing; Original Paper; Quantum dots; Quantum Dots - chemistry; Signal processing; Signal strength; Electrochemiluminescence biosensors; Molybdenum disulfide-graphene quantum dots; DNA walker; Signal amplification
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-021-04962-3

Based on the prominent electrochemiluminescence (ECL) performances of molybdenum disulfide-graphene quantum dots (MoS 2 -GQDs) nanocomposite and combined with enzyme-assisted recycling DNA walker signal amplification, an “on-off” switch ECL biosensor was proposed for sensitive assay of specific DNA sequences. Noticeably, MoS 2 with two-dimensional nanosheet structure increased the loading capacity of GQDs to support abundant hairpin DNA (H). The composites of MoS 2 and GQDs facilitated the charge transfer in ECL process, which significantly improved the ECL signal to achieve an “on” state. Then, the DNA walker cyclic amplification was performed by adding the target DNA and exonuclease III (Exo III). Finally, the DNA2-Fc-DNA1 was introduced into the system as ECL signal quencher, turning the ECL signal into an “off” state. The sensitive assay of ultra-low concentration specific DNA sequences was realized according to the variation of ECL signal strength before and after the existence of target DNA. The proposed ECL biosensor showed a good linear relationship ranging from 1 nM to 100 aM with a detection limit of 25.1 aM, providing a powerful strategy for biomedical research and clinical analysis. Graphical abstract