Silver-ion reversed-phase comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection in lipidic food analysis

• Published in: Journal of Chromatography A Vol. 1086; no. 1; pp. 91 - 98
• Main Authors: Mondello, L., Tranchida, P.Q., Stanek, Vaclav, Jandera, Pavel, Dugo, G., Dugo, P.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 09.09.2005; Elsevier
• Subjects: Analytical chemistry; APCI-MS; atmospheric pressure chemical ionization mass spectrometry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Comprehensive two-dimensional liquid chromatography; Exact sciences and technology; Fat industries; Food Analysis; Food industries; Fundamental and applied biological sciences. Psychology; lipids; Lipids - analysis; mass spectrometry; Mass Spectrometry - methods; Other chromatographic methods; reversed-phase liquid chromatography; rice oil; silver; TAG; Triacylglycerols; TAG; APCI-MS; Comprehensive two-dimensional liquid chromatography; Triacylglycerols; Chemical analysis; Two dimensional chromatography; Atmospheric pressure; Coupled method; HPLC chromatography; Lipids; Silver Ions; Edible oil; Fatty acids; Glyceride; Chemical ionization; Reversed phase chromatography; Triacylglycerol; Chemical composition; Rice bran oil; Mass spectrometry
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2005.06.017

The triacylglycerol (TAG) profiles present in real world lipidic samples are usually quite complex and, as such, monodimensional high-performance liquid chromatographic (HPLC) techniques are inadequate when challenged with such matrices. In this respect, the complementary use of silver-ion (Ag) and non-aqueous (NA) reversed-phase (RP) HPLC can be exploited if thorough TAG separations are required. The present investigation reports the employment of a newly developed comprehensive LC (LC × LC) system, based on the different separation mechanisms of the aforementioned techniques, and applied to a rice oil sample. The approach was successful in the separation of a high number of solutes, otherwise unachievable through monodimensional LC. Furthermore, the use of atmospheric pressure chemical ionization mass spectrometry (APCI-MS), as detection system, provided a third analytical dimension boosting the identification power of the comprehensive chromatographic method.