Inhibition of endoplasmic reticulum stress in high-fat-diet-induced obese C57BL/6 mice: Efficacy of a novel extract from mulberry ( Morus alba ) leaves fermented with Cordyceps militaris

• Published in: Laboratory animal research Vol. 34; no. 4; pp. 288 - 294
• Main Authors: Lee, Mi Rim, Bae, Su Ji, Kim, Ji Eun, Song, Bo Ram, Choi, Jun Young, Park, Jin Ju, Park, Ji Won, Kang, Mi Ju, Choi, Hyeon Jun, Choi, Young Whan, Kim, Kyung Mi, Hwang, Dae Youn
• Format: Journal Article
• Language: English
• Published: England Korean Association for Laboratory Animal Science 01.12.2018; BMC; 한국실험동물학회
• Subjects: apoptosis; ER stress response; fatty liver; Mulberry leaf; Original; 수의학; apoptosis; Mulberry leaf; fatty liver; ER stress response
• ISSN: 1738-6055; 2233-7660
• DOI: 10.5625/lar.2018.34.4.288

A few clues about correlation between endoplasmic reticulum (ER) stress and mulberry ( ) leaves were investigated in only the experimental autoimmune myocarditis and streptozotocin-induced diabetes. To investigate whether a novel extract of mulberry leaves fermented with (EMfC) could suppress ER in fatty liver, alterations in the key parameters for ER stress response were measured in high fat diet (HFD)-induced obese C57L/6 mice treated with EMfC for 12 weeks. The area of adipocytes in the liver section were significantly decreased in the HFD+EMfC treated group as compared to the HFD+Vehicle treated group, while their level was higher in HFD+Vehicle treated group than No treated group. The level of the eukaryotic initiation factor 2 alpha (eIF2α) and inositol-requiring enzyme 1 beta (IRE1α) phosphorylation and CCAAT-enhancer-binding protein homologous protein (CHOP) expression were remarkably enhanced in the HFD+Vehicle treated group. However, their levels were restored in the HFD+EMfC treated group, although some differences were detected in the decrease rate. Similar recovery was observed on the ER stress-induced apoptosis. The level of Caspase-3, Bcl-2 and Bax were decreased in the HFD+EMfC and HFD+orlistat (OT) treated group compared to the HFD+Vehicle treated group. The results of the present study therefore provide first evidence that EMfC with the anti-obesity effects can be suppressed ER stress and ER stress-induced apoptosis in the hepatic steatosis of HFD-induced obesity model.