Determination of glyphosate and its metabolites in plant material by reversed-polarity CE with indirect absorptiometric detection
A simple CE method for simultaneous determination of glyphosate and its metabolites (i.e. aminomethylphosphonic acid, glyoxylate, sarcosine and formaldehyde) in plants is reported here. A BGE of pH 7.5, 10% ACN, 7.5 mM phthalate, containing 0.75 mM hexadecyltrimethylammonium bromide as an electro‐os...
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Published in | Electrophoresis Vol. 31; no. 8; pp. 1423 - 1430 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.04.2010
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | A simple CE method for simultaneous determination of glyphosate and its metabolites (i.e. aminomethylphosphonic acid, glyoxylate, sarcosine and formaldehyde) in plants is reported here. A BGE of pH 7.5, 10% ACN, 7.5 mM phthalate, containing 0.75 mM hexadecyltrimethylammonium bromide as an electro‐osmotic flow modifier, an applied voltage of –20 kV and absorptiometric monitoring at 220 nm were the optimal chemical and instrumental parameters. The method, with development time 20 min, shows linear calibrations within the range 5–500 μg/mL (for all target analytes) with correlation coefficients between 0.999 and 0.998. It has been validated by application to samples of Lolium spp. The electroinjection mode hinders most interferents to enter the capillary, thus providing a clean electropherogram and making unnecessary long sample‐preparation steps. |
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Bibliography: | ark:/67375/WNG-2NQPJW1K-C ArticleID:ELPS200900583 istex:2938F6C55A12ABBE43EA961002382A7BDFCDD2E4 Spanish Ministerio de Ciencia e Innovación (MICINN) - No. AGL-2007 60771; No. CTQ2009-07430 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/elps.200900583 |