Isolation of a GPD gene from Debaryomyces hansenii encoding a glycerol 3‐phosphate dehydrogenase (NAD+)

• Published in: Yeast (Chichester, England) Vol. 21; no. 2; pp. 119 - 126
• Main Author: Thome, P E
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 30.01.2004
• Subjects: Amino Acid Sequence; Base Sequence; Blotting, Southern; Debaryomyces hansenii; DNA Fingerprinting; DNA, Fungal - genetics; DNA, Fungal - isolation & purification; DNA, Fungal - metabolism; Genes, Fungal; Genetic Complementation Test; glycerol 3‐phosphate dehydrogenase; Glycerol-3-Phosphate Dehydrogenase (NAD+); Glycerolphosphate Dehydrogenase - genetics; GPD1; Molecular Sequence Data; Mutagenesis, Insertional; osmoregulation; Saccharomyces cerevisiae - genetics; Saccharomycetales - enzymology; Saccharomycetales - genetics; salt‐stress; Sequence Alignment; yeast
• ISSN: 0749-503X; 1097-0061
• DOI: 10.1002/yea.1070

A gene homologous to GPD1, coding for glycerol‐3‐phosphate dehydrogenase (sn‐glycerol 3‐phosphate: NAD+ oxidoreductase, EC 1.1.1.8), has been isolated from the halophilic yeast Debaryomyces hansenii by complementation of a Saccharomyces cerevisiae gpd1Δ mutant. DNA sequencing of the complementing genomic clone indicated the existence of an open reading frame encoding a protein with 369 amino acids. Comparative analysis of the deduced amino acid sequence showed high similarity to homologous genes described for other eukaryotic GPD enzymes. The sequence has been submitted to the GenBank database under Accession No. AY333427. Copyright © 2003 John Wiley & Sons, Ltd.