Catalytic removal of sulfide by an immobilized sulfide-oxidase bioreactor

• Published in: Enzyme and microbial technology Vol. 44; no. 2; pp. 96 - 100
• Main Authors: Zhang, Chun-Ming, Luan, Xing-She, Xiao, Min, Song, Jian, Lu, Lili, Xiao, Xiao
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 05.02.2009; Elsevier
• Subjects: Biological and medical sciences; Bioreactor; Bioreactors; Biotechnology; Catalytic removal; Fundamental and applied biological sciences. Psychology; Immobilized sulfide-oxidase; Methods. Procedures. Technologies; Streptomyces; Streptomyces sp; Sulfide; Various methods and equipments; Sulfide; Bioreactor; Catalytic removal; Streptomyces sp; Immobilized sulfide-oxidase; Streptomycetaceae; Enzyme; Oxidase; Streptomyces; Actinomycetales; Sulfides; Bacteria; Actinomycetes; Oxidoreductases
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/j.enzmictec.2008.10.010

A bioreactor packed with chitosan immobilized sulfide-oxidase from Streptomyces species LD048 was developed to treat a liquid stream of sulfide. The inoculation system was composed of glass with a 0.7 L working volume and enzyme activity of 2 mmol S g −1 carrier. The sulfide removal efficiency was almost 100% when the volumetric loading was increased up to 3.9 mmol S L −1 h −1 at a space velocity of 18 h −1. The maximal elimination capacity was 22.1 mmol S L −1 h −1 with a space velocity of 72 h −1. When the aeration was increased from 0.05 to 0.1 L min −1, the average removal efficiency improved from 81% to 94%. A removal efficiency of 90% was obtained after 15 days of operation with a load rate of 8.9 mmol S L −1 h −1 and a space velocity of 14.28 h −1. An operational equation based on the ideal plug flow bioreactor and the Michaelis–Menten model predicted the performance of this bioreactor.