Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course in patients with psoriasis

• Published in: International journal of occupational medicine and environmental health Vol. 33; no. 5; pp. 635 - 647
• Main Authors: Kutwin, Magdalena, Migdalska-Sęk, Monika, Brzeziańska-Lasota, Ewa, Zelga, Piotr, Woźniacka, Anna
• Format: Journal Article
• Language: English
• Published: Poland Nofer Institute of Occupational Medicine 17.09.2020
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers - analysis; disease activity; Female; Humans; immunology; Interferon-gamma - genetics; Interferon-gamma - immunology; Interferon-gamma - therapeutic use; Interleukin-12 - genetics; Interleukin-12 - immunology; Interleukin-12 - therapeutic use; Interleukin-22; Interleukins - genetics; Interleukins - immunology; Interleukins - therapeutic use; Male; Middle Aged; molecular markers; Poland - epidemiology; proinflammatory cytokines; psoriasis; Psoriasis - drug therapy; Psoriasis - epidemiology; Psoriasis - genetics; Psoriasis - immunology; skin disease; Young Adult; Poland; disease activity; skin disease; proinflammatory cytokines; Psoriasis; immunology; molecular markers
• ISSN: 1232-1087; 1896-494X; 1896-494X
• DOI: 10.13075/ijomeh.1896.01513

As a chronic, recurrent, immunologically mediated systemic disease and a common cause of dermatological problems, psoriasis is often a subject of scientific research. Skin changes located on the hands can cause difficulties and limitations in the performance of professional activities, especially manual ones. The main role in pathogenesis is played by immunological factors - improper functioning of the components of the immune system, among others, T lymphocytes and cytokines like interleukin-12 (IL-12), interleukin-22 (IL-22) and interferon gamma (IFN-γ). The obtained tissue and blood were destined for RNA isolation. The RNA was then subjected to a reverse transcription reaction. The relative gene expression level was evaluated by the real-time polymerase chain reaction for , and genes, and presented as the relative quantification (RQ) value, relative to the reference gene . In addition, a correlation analysis of the expression level of selected genes with the clinical course of the disease, as assessed by the (PASI), the (BSA) and the (DLQI) scores was performed. Statistical analysis confirmed a significant increase in RQ values for , and in the group of psoriatic patients vs. the control group. A positive correlation was also found between BSA and PASI and RQ for the gene. Increased expression levels of , and genes in psoriatic skin confirm that selected cytokines play an important role in the initiation and sustenance of psoriasis. Int J Occup Med Environ Health. 2020;33(5):635-47.