P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migration into inflamed skin

• Published in: The Journal of experimental medicine Vol. 185; no. 3; pp. 573 - 578
• Main Authors: Borges, E, Tietz, W, Steegmaier, M, Moll, T, Hallmann, R, Hamann, A, Vestweber, D
• Format: Journal Article
• Language: English
• Published: United States The Rockefeller University Press 03.02.1997
• Subjects: AIDS/HIV; Animals; Brief Definitive Report; Cell Movement; Cells, Cultured; Dermatitis - immunology; Membrane Glycoproteins - physiology; Mice; Mice, Inbred BALB C; P-Selectin - metabolism; Th1 Cells - physiology; Th2 Cells - physiology
• ISSN: 0022-1007; 1540-9538
• DOI: 10.1084/jem.185.3.573

We have shown recently that mouse Th1 cells but not Th2 cells are selectively recruited into inflamed sites of a delayed-type hypersensitivity (DTH) reaction of the skin. This migration was blocked by monoclonal antibodies (mAb) against P- and E-selectin. Here we show that Th1 cells bind to P-selectin via the P-selectin glycoprotein ligand-1 (PSGL-1). This is the only glycoprotein ligand that was detectable by affinity isolation with a P-selectin-Ig fusion protein. Binding of Th1 cells to P-selectin, as analyzed by flow cytometry and in cell adhesion assays, was completely blocked by antibodies against PSGL-1. The same antibodies blocked partially the migration of Th1 cells into cutaneous DTH reactions. This blocking activity, in combination with that of a mAb against E-selectin, was additive. PSGL-1 on Th2 cells, although expressed at similar levels as on Th1 cells, did not support binding to P-selectin. Thus, the P-selectin-binding form of PSGL-1 distinguishes Th1 cells from Th2 cells. Furthermore, PSGL-1 is relevant for the entry of Th1 cells into inflamed areas of the skin. This is the first demonstration for the importance of PSGL-1 for mouse leukocyte recruitment in vivo.