High-throughput screening for gene libraries expressing carbohydrate hydrolase activity

A simple and fast method is described allowing screening of large number of Escherichia coli clones (4000 per day) for the presence of functional or improved carbohydrate hydrolase enzymes. The procedure is relatively cheap and has the advantage that carbohydrate degrading activity can be directly m...

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Bibliographic Details
Published inBiotechnology letters Vol. 25; no. 19; pp. 1643 - 1645
Main Authors Leemhuis, H, Euverink, G.J.W, Dijkhuizen, L
Format Journal Article
LanguageEnglish
Published Netherlands Springer Nature B.V 01.10.2003
Subjects
Bacteria
carbohydrate hydrolase
Carbohydrate Metabolism
clones
Cloning, Molecular
DNA libraries
E coli
Enzyme Activation
enzymes
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli - isolation & purification
Gene Expression Profiling - methods
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Gene Library
Glucosyltransferases - genetics
Glucosyltransferases - metabolism
high-throughput screening
Hydrolases - metabolism
Microbiology
Mutation
screening
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Summary:A simple and fast method is described allowing screening of large number of Escherichia coli clones (4000 per day) for the presence of functional or improved carbohydrate hydrolase enzymes. The procedure is relatively cheap and has the advantage that carbohydrate degrading activity can be directly measured using liquid cultures grown in microtiter plates without the need of separation or purification steps.
Bibliography:http://www.kluweronline.com/issn/0141-5492/contents
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ISSN:0141-5492
1573-6776
DOI:10.1023/A:1025646807840