MDM2 SNP 309 human papillomavirus infection in cervical carcinogenesis

• Published in: Gynecologic oncology Vol. 118; no. 3; pp. 258 - 261
• Main Authors: Nunobiki, Osamu, Ueda, Masatsugu, Yamamoto, Michiko, Toji, Eisaku, Sato, Naomi, Izuma, Shinji, Okamoto, Yoshiaki, Torii, Kiyo, Noda, Sadamu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2010
• Subjects: Alleles; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Carcinoma, Squamous Cell - virology; Cell Line, Tumor; Cell Transformation, Viral - genetics; Cervical carcinogenesis; Cervical Intraepithelial Neoplasia - genetics; Cervical Intraepithelial Neoplasia - pathology; Cervical Intraepithelial Neoplasia - virology; Female; Hematology, Oncology and Palliative Medicine; HPV; Humans; MDM2; Obstetrics and Gynecology; Papillomaviridae - genetics; Papillomavirus Infections - genetics; Papillomavirus Infections - pathology; Polymerase Chain Reaction; Polymorphism; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Proto-Oncogene Proteins c-mdm2 - genetics; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - virology; MDM2; Cervical carcinogenesis; Polymorphism; HPV
• ISSN: 0090-8258; 1095-6859
• DOI: 10.1016/j.ygyno.2010.05.009

Abstract Objective To investigate the biological significance of single nucleotide polymorphism (SNP) at murine double-minute 2 homolog (MDM2) promoter 309 in cervical carcinogenesis. Methods SNP at MDM2 promoter 309 (T/G) together with human papillomavirus (HPV) types was examined in a total of 195 cervical smear samples and 8 human cervical squamous carcinoma cell lines using two independent PCR assays and PCR-RFLP techniques. Results Forty-one patients with high-grade squamous intraepithelial lesion (HSIL) had higher frequency of high-risk HPV than 102 with low-grade SIL (LSIL) and 52 controls. There was an increased OR (8.88; CI = 2.34–33.63; P = 0.003) for TG + GG genotype in HSIL cases compared to controls among 68 patients with high-risk HPV. Twenty-one cases with HPV types 16 and/or 18 had significantly higher frequency of the TG + GG genotype and G allele than 47 with other types of high-risk HPV. Seven of 8 cervical carcinoma cell lines also showed TG or GG genotype. Conclusion MDM2-SNP309 (T/G) and high-risk HPV infection may be closely associated with cervical carcinogenesis in a Japanese population.