Encapsulation of thiosulfate: cyanide sulfurtransferase by mouse erythrocytes

Murine carrier erythrocytes, prepared by hypotonic dialysis, were employed in the encapsulation of several compounds including [14C]sucrose, [3H]inulin, and bovine thiosulfate:cyanide sulfurtransferase (rhodanese), a mitochondrial enzyme which converts cyanide to thiocyanate. Approximately 30% of th...

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Published inToxicology and applied pharmacology Vol. 83; no. 1; p. 101
Main Authors Leung, P, Ray, L E, Sander, C, Way, J L, Sylvester, D M
Format Journal Article
LanguageEnglish
Published United States 30.03.1986
Subjects
Animals
Carbon Radioisotopes
Cyanides - metabolism
Dialysis
Erythrocytes - enzymology
Inactivation, Metabolic
Inulin - blood
Male
Mice
Mice, Inbred BALB C
Osmolar Concentration
Sucrose - blood
Sulfurtransferases - blood
Thiosulfate Sulfurtransferase - administration & dosage
Thiosulfate Sulfurtransferase - blood
Tritium
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Summary:Murine carrier erythrocytes, prepared by hypotonic dialysis, were employed in the encapsulation of several compounds including [14C]sucrose, [3H]inulin, and bovine thiosulfate:cyanide sulfurtransferase (rhodanese), a mitochondrial enzyme which converts cyanide to thiocyanate. Approximately 30% of the added [14C]sucrose, [3H]inulin, and rhodanese was encapsulated by predialyzed erythrocytes, and a decrease in the mean corpuscular volume and mean corpuscular hemoglobin was observed. In the encapsulation of rhodanese a recovery of 95% of the erythrocytes was achieved and an 85% equilibrium was established. The addition of potassium cyanide (50 mM) to intact, rhodanese-loaded erythrocytes containing sodium thiosulfate resulted in its metabolism to thiocyanate. These results establish the potential use of erythrocytes as biodegradable drug carrier in drug antagonism.
ISSN:0041-008X
DOI:10.1016/0041-008X(86)90327-3