Antiarthritic and antiinflammatory propensity of 4-methylesculetin, a coumarin derivative

• Published in: Biochimie Vol. 95; no. 6; pp. 1326 - 1335
• Main Authors: Hemshekhar, M., Sunitha, K., Thushara, R.M., Sebastin Santhosh, M., Shanmuga Sundaram, M., Kemparaju, K., Girish, K.S.
• Format: Journal Article
• Language: English
• Published: France Elsevier B.V 01.06.2013
• Subjects: 4-Methylesculetin; Animals; Anti-Inflammatory Agents - pharmacology; Arthritis; Arthritis, Experimental - drug therapy; Arthritis, Experimental - pathology; Bone and Bones - drug effects; Bone and Bones - pathology; Cartilage - drug effects; Cartilage - pathology; Cartilage degradation; Coumarins - chemistry; Disease Models, Animal; Inflammation - pathology; Inflammatory mediators; Male; Oxidative stress; Oxidative Stress - drug effects; Rats; Rats, Wistar; Umbelliferones - pharmacology; Oxidative stress; HVA; 4-Methylesculetin; DCF; MMPs; HAases; ROS; Arthritis; Inflammatory mediators; Cartilage degradation
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/j.biochi.2013.02.014

Coumarins are a group of natural compounds widely distributed in plants. Of late, coumarins and their derivatives have grabbed much attention from the pharmacological and pharmaceutical arena due to their broad range of therapeutical qualities. A coumarin derivative 4-methylesculetin (4-ME) has known to possess effective antioxidant and radical-scavenging properties. Recently they have also shown to down regulate nuclear factor-kappa B (NF-κB) and protein kinase B (Akt) that play a vital role in inflammation and apoptosis. In view of this, the present study investigated the anti-arthritic potentiality of 4-ME by assessing its ability to inhibit cartilage and bone degeneration, inflammation and associated oxidative stress. Arthritis being a debilitating joint disease, results in the deterioration of extracellular matrix (ECM) of cartilage and synovium. Participation of both enzymatic and non-enzymatic factors in disease perpetuation is well documented. The present study demonstrated the mitigation of augmented serum levels of hyaluronidase and matrix metalloproteinases (MMP-13, MMP-3 and MMP-9) responsible for cartilage degeneration by 4-ME. It also protected bone resorption by reducing the elevated levels of bone-joint exoglycosidases, cathepsin-D and tartrate resistant acid phosphatases. Further, 4-ME significantly ameliorated the upregulated non-enzymatic inflammatory markers like TNF-α, IL-1β, IL-6, COX-2 and PGE2. Besides, 4-ME effectively stabilized the arthritis-induced oxidative stress by restoring the levels of reactive oxygen species, lipid and hydro peroxides and antioxidant enzymes such as superoxide dismutase, catalase and glutathione-S-transferase. Thus, the study suggests that 4-ME could be an effective agent to treat arthritis and associated secondary complications like oxidative stress. ► First report on the antiarthritic propensity of 4-methylesculetin. ► 4-Methylesculetin prevents cartilage and bone degeneration. ► 4-Methylesculetin alleviate arthritis induced oxidative stress in liver and spleen. ► 4-Methylesculetin quenches arthritis induced inflammatory response. ► Treatment with 4-methylesculetin restores joint homeostasis in arthritic animals.