Human teratocarcinoma cells express functional insulin-like growth factor I receptors

• Published in: Experimental cell research Vol. 184; no. 2; pp. 427 - 439
• Main Authors: Weima, Sjerp M., Stet, Let H., Van Rooijen, Marga A., Van Buul-Offers, Sylvia C., Van Zoelen, Everardus J.J., De Laat, Siegfried W., Mummery, Christine L.
• Format: Journal Article
• Language: English
• Published: Orlando, FL Elsevier Inc 01.10.1989; Elsevier
• Subjects: 550301 - Cytology- Tracer Techniques; ANIMAL CELLS; ANIMALS; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; Biological and medical sciences; CARCINOMAS; Cell Line; Cell Membrane - metabolism; Cell Membrane - ultrastructure; CELL PROLIFERATION; Cell receptors; Cell structures and functions; Cell Transformation, Neoplastic - drug effects; CHEMICAL REACTIONS; CROSS-LINKING; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; EMBRYONIC CELLS; ENZYMES; Fundamental and applied biological sciences. Psychology; Gene Expression; GLYCOPROTEINS; GROWTH FACTORS; Humans; Insulin - pharmacology; Insulin-Like Growth Factor I - pharmacology; Insulin-Like Growth Factor II - pharmacology; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE 125; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MAN; MEMBRANE PROTEINS; MITOGENS; Molecular and cellular biology; NEOPLASMS; Neoplasms, Germ Cell and Embryonal - metabolism; Neoplasms, Germ Cell and Embryonal - pathology; Neoplasms, Germ Cell and Embryonal - ultrastructure; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PHOSPHOTRANSFERASES; Phosphotransferases - metabolism; POLYMERIZATION; PRIMATES; PROTEINS; RADIOISOTOPES; RADIORECEPTOR ASSAY; RECEPTORS; Receptors, Cell Surface - genetics; Receptors, Cell Surface - physiology; Receptors, Somatomedin; Teratoma - metabolism; Teratoma - pathology; Teratoma - ultrastructure; TRACER TECHNIQUES; TRANSFERASES; Tretinoin - pharmacology; TUMOR CELLS; Tumor Cells, Cultured - metabolism; Tumor Cells, Cultured - pathology; VERTEBRATES; Human; Cell culture; Ligand binding; Radiolabelling; Somatomedin C; Enzyme; Crosslinking; Activation; Monoclonal antibody; Malignant teratoma; Insulin like growth factor 2; Insulin; Immunological method; Membrane receptor; Enzymatic activity; Affinity chromatography; Scatchard plot; Protein-tyrosine kinase
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/0014-4827(89)90341-8

Using iodinated insulin-like growth factors (IGFs) we have detected receptors for IGF-I at the cell surface of the clonally derived human embryonal carcinoma cell line Tera 2 clone 13. Affinity crosslinking of IGFs to Tera 2 clone 13-derived membrane preparations revealed the presence of proteins with features of both type-I and type-II IGF receptors. Treatment of Tera 2 clone 13 cells with retinoic acid to induce differentiation results in an increased number of cell surface receptors, apparently without altering the ratio of type-I and type-II receptors. In addition, Tera 2 clone 13 IGF-I receptors catalyze (auto)phosphorylation at tyrosine upon IGF-I and insulin binding. These findings suggest that type-I IGF receptors might be involved in mediating the effects of IGFs and insulin upon the proliferation of Tera 2 clone 13 cells.