In vivo flow speed measurement of capillaries by photoacoustic correlation spectroscopy

We recently proposed photoacoustic correlation spectroscopy (PACS) and demonstrated a proof-of-concept experiment. Here we use the technique for in vivo flow speed measurement in capillaries in a chick embryo model. The photoacoustic microscopy system is used to render high spatial resolution and hi...

Full description

Saved in:
Bibliographic Details
Published inOptics letters Vol. 36; no. 20; p. 4017
Main Authors Chen, Sung-Liang, Xie, Zhixing, Carson, Paul L, Wang, Xueding, Guo, L Jay
Format Journal Article
LanguageEnglish
Published United States 15.10.2011
Subjects
Animals
Blood Circulation - physiology
Blood Flow Velocity
Calibration
Capillaries - physiology
Chick Embryo
Erythrocytes - physiology
Phantoms, Imaging
Sensitivity and Specificity
Spectrometry, Fluorescence - methods
Online AccessGet more information

Cover

More Information
Summary:We recently proposed photoacoustic correlation spectroscopy (PACS) and demonstrated a proof-of-concept experiment. Here we use the technique for in vivo flow speed measurement in capillaries in a chick embryo model. The photoacoustic microscopy system is used to render high spatial resolution and high sensitivity, enabling sufficient signals from single red blood cells. The probe beam size is calibrated by a blood-mimicking phantom. The results indicate the feasibility of using PACS to study flow speeds in capillaries.
ISSN:1539-4794
DOI:10.1364/OL.36.004017