Lanthanide ion enhancement of interferon binding to cells

• Published in: Biochemical and biophysical research communications Vol. 137; no. 1; pp. 480 - 485
• Main Authors: Sedmak, J.James, MacDonald, Hector S., Kushnaryov, Vladimir M.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 29.05.1986; Elsevier
• Subjects: Animals; Applied sciences; Cell Line; Exact sciences and technology; Humans; Interferon Type I - metabolism; Lanthanum - pharmacology; Mice; Other techniques and industries; Protein Binding - drug effects; Receptors, Immunologic - metabolism; Receptors, Interferon; Species Specificity; Ytterbium - pharmacology
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(86)91235-0

Pretreatment of purified [ 125I]-labeled human and mouse β interferons (IFN) with lanthanum chloride (LaCl 3) enhanced 20–30 fold the binding of the [ 125I]-IFNs to human A549 and mouse L cells at 0°C and also enhanced antiviral activity in homologous cells. Although lanthanides enhanced cross-species binding of both human and mouse [ 125I]-IFNs, there was no increase in cross-species antiviral activity. Unlabeled IFN not treated with LaCl 3 did not compete with [ 125I]-IFN treated with LaCl 3 for cellular receptors. However, unlabeled IFN treated with LaCl 3 did compete with LaCl 3-treated [ 125I]-IFN. These results suggest that lanthanide treated IFNs do not bind to the same receptors as native IFNs.