Dose-Dependent Effect of Green Tea (Camellia sinensis) Extract in the Post-Thawed Fertility Indicators of frozen Bovine Semen Extender

ABSTRACT This study describes antioxidative activity of green tea (GT) extract on standard post-thawed quality indicators of frozen buffalo bull's spermatozoa. Semen samples were obtained from the bulls for three weeks through artificial vagina. Egg yolk was used as an extender, in which differ...

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Published inPakistan journal of zoology Vol. 54; no. 2; p. 647
Main Authors Khattak, Noor Bad Shah, Khan, Hamayun, Tunio, Muhammad Tarique, Ahmad, Shakoor, Ahmad, Nazir, Andrabi, Syed Murtaza Hassan, Obaidullah, Ali, Ihsan, Fang, Nan-Zhu
Format Journal Article
LanguageEnglish
Published Lahore Knowledge Bylanes 30.04.2022
AsiaNet Pakistan (Pvt) Ltd
Subjects
Antioxidants
Artificial insemination
Buffalo
Bulls
Cattle
Chemical properties
Cryopreservation
Cryopreservation of organs, tissues, etc
Cytotoxicity
Fertility
Freeze-thaw
Freezing
Green tea
Indicators
Integrity
Laboratories
Lipid peroxidation
Melting
Membranes
Motility
Oxidative stress
Physiological aspects
Plasma
Polyphenols
Semen
Sperm
Spermatozoa
Survivability
Tea
Thawing
Yolk
Pakistan
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Summary:ABSTRACT This study describes antioxidative activity of green tea (GT) extract on standard post-thawed quality indicators of frozen buffalo bull's spermatozoa. Semen samples were obtained from the bulls for three weeks through artificial vagina. Egg yolk was used as an extender, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo bull sperms. The antioxidative effect of GT was observed at the comparatively lower concentration (0.1-1.5%) on the post-thawed quality indicators. On the other hand, the hight GT concentration (1.5%) significantly decreased the quality indicators of spermatozoa. Green tea extract enhanced the spermatozoa survivability, integrity of acrosome and plasma membrane of spermatozoa following freezing and thawing. It is suggested that the addition of 1.5% green tea extract (4 g in 200 ml methanol) can be safely used for enhanced effectiveness of fertility after thawing.
ISSN:0030-9923
DOI:10.17582/journal.pjz/20181230051216