genetic structure of Drosophila subobscura populations from the islands of Majorca and Minorca (Balearic Islands, Spain) based on allozymes and mitochondrial DNA

• Published in: Heredity Vol. 83; no. 3; pp. 271 - 279
• Main Authors: Castro, J.A, Ramon, M, Picornell, A, Moya, A
• Format: Journal Article
• Language: English
• Published: England Springer Nature B.V 01.09.1999
• Subjects: Acph-1 gene; Alleles; allozymes; Animals; DNA, Mitochondrial; Drosophila - enzymology; Drosophila - genetics; Drosophila subobscura; Enzymes - genetics; Female; Gene Frequency; Genetic structure; Genetics, Population; Haplotypes; Islands; isozymes; loci; Mitochondrial DNA; Natural populations; Phylogeny; restriction mapping; Balearic Islands
• ISSN: 0018-067X; 1365-2540
• DOI: 10.1038/sj.hdy.6885500

The genetic structure of seven populations of Drosophi1a subobscura from different locations on Majorca and Minorca (Balearic Islands, Spain) was studied using two types of markers: allozyme and mitochondrial DNA restriction analyses. Both markers showed congruent results. In the allozyme data, when the Acph-1 locus was excluded from the joint F(ST) statistics, only three out of 21 comparisons were statistically significant, lending support to the hypothesis of low genetic differentiation. The mtDNA restriction analyses showed two haplotypes at a high frequency (more than 40% each), irrespective of the location considered, and a number of endemic haplotypes at very low frequencies (not higher than 2% each). The analyses of the genetic structure yielded a pattern similar to the allozymes. The cytonuclear disequilibrium analyses showed the difficulty of detecting cytonuclear associations in natural populations because they are mainly transient.