Prevalence and genotypes of Enterocytozoon bieneusi in pigs in southern China

• Published in: Infection, genetics and evolution Vol. 66; pp. 52 - 56
• Main Authors: Zou, Yang, Hou, Jun-Ling, Li, Fa-Cai, Zou, Feng-Cai, Lin, Rui-Qing, Ma, Jian-Gang, Zhang, Xiao-Xuan, Zhu, Xing-Quan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2018
• Subjects: Enterocytozoon bieneusi; Genotypes; Multilocus sequence typing (MLST); Pigs; Prevalence; Southern China; Pigs; Prevalence; Southern China; Genotypes; Multilocus sequence typing (MLST); Enterocytozoon bieneusi
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2018.09.006

Enterocytozoon bieneusi is one of the most important causative agents of microsporidiosis, causing diarrhoea the symptoms of enteric disease in humans and animals. Although there is some information on the prevalence and genotypes of E. bieneusi in China, there is still a lack of data in pigs in southern China. In the present study, a total of 396 faecal specimens were collected from pigs in Zhejiang, Guangdong and Yunnan provinces in southern China, and were examined by nested PCR amplification of the ribosomal internal transcribed spacer (ITS) for the prevalence and genotypes of E. bieneusi. The overall prevalence of E. bieneusi in pigs was 31.57% (125/396), forming 15 genotypes, including 9 known genotypes (EbpC, EbpA, D, G, H, PigEBITS5, Henan-IV, KIN-1, CHS5) and 6 novel genotypes (GD1, ZJ1, ZJ2, YN1, YN2 and YN3), which were all clustered into Group 1. Moreover, multilocus sequence typing (MLST) showed that 6, 3, 4 and 5 types were identified in MS1, MS3, MS7 and MS4 loci, respectively, representing four multilocus genotypes (MLGs), designated as MLGs novel-1 to novel-4 in the present study. This is the first detailed study of E. bieneusi using MLST in pigs in southern China, which extended information about the distribution of E. bieneusi genotypes in China. •E. bieneusi prevalence and genotypes in pigs were examined by nested PCR of the ribosomal internal transcribed spacer•15 genotypes, including 6 novel genotypes of E. bieneusi were identified in pigs•E. bieneusi positive samples were characterized by multilocus sequence typing•Four new multilocus genotypes (MLGs novel-1-novel-4) were observed in pigs•This study extended the information of distribution of E. bieneusi genotypes in China