Comparison of the In Vitro and In Vivo Toxic Effects of Three Sizes of Zinc Oxide (ZnO) Particles Using Flounder Gill (FG) Cells and Zebrafish Embryos

• Published in: Journal of Ocean University of China Vol. 16; no. 1; pp. 93 - 106
• Main Authors: Han, Li, Zhai, Yanan, Liu, Yang, Hao, Linhua, Guo, Huarong
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.02.2017; Springer Nature B.V; Key Laboratory of Marine Genetics and Breeding(Ocean University of China),Ministry of Education,College of Marine Life Sciences,Qingdao 266003,P.R.China%First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,P.R.China%Key Laboratory of Marine Genetics and Breeding(Ocean University of China),Ministry of Education,College of Marine Life Sciences,Qingdao 266003,P.R.China; Institute of Evolution and Marine Biodiversity,Ocean University of China,Qingdao 266003,P.R.China
• Subjects: Aquatic organisms; Bioassays; cells; Cellular biology; Cytotoxicity; Danio rerio; Earth and Environmental Science; Earth Sciences; Embryos; embryotoxicity; Environmental release; Fish; Freshwater; Hatching; Marine; Meteorology; Nanoparticles; Oceanography; oxide; Pleuronectiformes; zebrafish; zinc; Zinc oxides; ZnO; zinc oxide (ZnO); embryotoxicity; fish cells; nanoparticles; zebrafish; cytotoxicity
• ISSN: 1672-5182; 1993-5021; 1672-5174
• DOI: 10.1007/s11802-017-2940-5

Nano-sized zinc oxide (nZnO) particles are one kind of the most commonly used metal oxide nanoparticles (NPs). This study compared the cytotoxic and embryotoxic effects of three increasing sized ZnO particles (30 nm, 80?150 nm and 2 μm) in the flounder gill (FG) cells and zebrafish embryos, and analyzed the contribution of size, agglomeration and released Zn2+ to the toxic effects. All the tested ZnO particles were found to be highly toxic to both FG cells and zebrafish embryos. They induced growth inhibition, LDH release, morphological changes and apoptosis in FG cells in a concentration-, size- and time-dependent manner. Moreover, the release of LDH from the exposed FG cells into the medium occurred before the observable morphological changes happened. The ultrasonication treatment and addition of serum favored the dispersion of ZnO particles and alleviated the agglomeration, thus significantly increased the corresponding cytotoxicity. The released Zn2+ ions from ZnO particles into the extracellular medium only partially contributed to the cytotoxicity. All the three sizes of ZnO particles tested induced developmental malformations, decrease of hatching rates and lethality in zebrafish embryos, but size- and concentration- dependent toxic effects were not so obvious as in FG cells possibly due to the easy aggregation of ZnO particles in freshwater. In conclusion, both FG cells and zebrafish embryos are sensitive bioassay systems for safety assessment of ZnO particles and the environmental release of ZnO particles should be closely monitored as far as the safety of aquatic organisms is concerned.