High-throughput species identification of enterococci using pyrosequencing

Here we report the development and validation of an automated high-throughput pyrosequencing-based method for the reliable identification of isolated Enterococcus species. This method exploits the discrete species-specificity of hypervariable groES-EL spacer region and utilizes a universal dispensat...

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Published inJournal of microbiological methods Vol. 89; no. 3; pp. 174 - 178
Main Authors Zaheer, Rahat, Yanke, L. Jay, Church, Deirdre, Topp, Edward, Read, Ronald R., McAllister, Tim A.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.06.2012
Elsevier
Subjects
Animals
Automation - methods
Bacterial Proteins - genetics
Bacteriological methods and techniques used in bacteriology
Bacteriological Techniques - methods
Bacteriology
Biological and medical sciences
Chaperonins - genetics
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Enterococcus
Enterococcus - classification
Enterococcus - genetics
Fundamental and applied biological sciences. Psychology
groES-EL
High-Throughput Nucleotide Sequencing - methods
Humans
Microbiology
Molecular Sequence Data
Pyrosequencing
sequence analysis
species identification
Enterococcus
groES-EL
Pyrosequencing
Streptococcaceae
Lactic acid bacteria
Bacteria
Micrococcales
Identification
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Abstract Here we report the development and validation of an automated high-throughput pyrosequencing-based method for the reliable identification of isolated Enterococcus species. This method exploits the discrete species-specificity of hypervariable groES-EL spacer region and utilizes a universal dispensation order optimized for a wide range of Enterococcus species.
AbstractList Here we report the development and validation of an automated high-throughput pyrosequencing-based method for the reliable identification of isolated Enterococcus species. This method exploits the discrete species-specificity of hypervariable groES-EL spacer region and utilizes a universal dispensation order optimized for a wide range of Enterococcus species.
Author Church, Deirdre
Zaheer, Rahat
Topp, Edward
McAllister, Tim A.
Read, Ronald R.
Yanke, L. Jay
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Issue 3
Keywords Enterococcus
groES-EL
Pyrosequencing
Streptococcaceae
Lactic acid bacteria
Bacteria
Micrococcales
Identification
Language English
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Snippet Here we report the development and validation of an automated high-throughput pyrosequencing-based method for the reliable identification of isolated...
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SubjectTerms Animals
Automation - methods
Bacterial Proteins - genetics
Bacteriological methods and techniques used in bacteriology
Bacteriological Techniques - methods
Bacteriology
Biological and medical sciences
Chaperonins - genetics
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Enterococcus
Enterococcus - classification
Enterococcus - genetics
Fundamental and applied biological sciences. Psychology
groES-EL
High-Throughput Nucleotide Sequencing - methods
Humans
Microbiology
Molecular Sequence Data
Pyrosequencing
sequence analysis
species identification
Title High-throughput species identification of enterococci using pyrosequencing
URI https://dx.doi.org/10.1016/j.mimet.2012.03.012
https://www.ncbi.nlm.nih.gov/pubmed/22465481
https://search.proquest.com/docview/1010636989
Volume 89
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