High-throughput species identification of enterococci using pyrosequencing

• Published in: Journal of microbiological methods Vol. 89; no. 3; pp. 174 - 178
• Main Authors: Zaheer, Rahat, Yanke, L. Jay, Church, Deirdre, Topp, Edward, Read, Ronald R., McAllister, Tim A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.2012; Elsevier
• Subjects: Animals; Automation - methods; Bacterial Proteins - genetics; Bacteriological methods and techniques used in bacteriology; Bacteriological Techniques - methods; Bacteriology; Biological and medical sciences; Chaperonins - genetics; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Enterococcus; Enterococcus - classification; Enterococcus - genetics; Fundamental and applied biological sciences. Psychology; groES-EL; High-Throughput Nucleotide Sequencing - methods; Humans; Microbiology; Molecular Sequence Data; Pyrosequencing; sequence analysis; species identification; Enterococcus; groES-EL; Pyrosequencing; Streptococcaceae; Lactic acid bacteria; Bacteria; Micrococcales; Identification
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2012.03.012

Here we report the development and validation of an automated high-throughput pyrosequencing-based method for the reliable identification of isolated Enterococcus species. This method exploits the discrete species-specificity of hypervariable groES-EL spacer region and utilizes a universal dispensation order optimized for a wide range of Enterococcus species.