A unified procedure for the correction of liquid junction potentials in patch clamp experiments on endo- and plasma membranes

• Published in: Journal of experimental botany Vol. 48; no. Special; pp. 361 - 364
• Main Authors: Amtmann, Anna, Sanders, Dale
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford OXFORD UNIVERSITY PRESS 01.03.1997; Oxford University Press
• Subjects: Amplifiers; Biological and medical sciences; Cell membranes; Cell physiology; Electric potential; Electrodes; Experimental procedures; Fundamental and applied biological sciences. Psychology; General Principles: Techniques and Models; Intracellular membranes; Ion channels; Liquids; Pipettes; Plant physiology and development; Plasma membrane and permeation; Vacuoles; Vegetals; Ionic selectivity; Plasma membrane; Patch clamp method; Procedure; Ionic channel; Membrane potential; Corrections; Liquid junction potential
• DOI: 10.1093/jxb/48.Special_Issue.361
• ISSN: 0022-0957

Correct determination of absolute values and polarities of liquid junction potentials (LJPs) is essential in patch clamp experiments for the estimation of ionic selectivities and activation voltages of ion channels. A simple approach for the correction of membrane voltages for LJPs has been developed. The method covers all combinations of LJPs between the solutions of bath, patch pipette and reference salt bridge and is applicable to all patch configurations on plasma membranes and endomembranes.