A unified procedure for the correction of liquid junction potentials in patch clamp experiments on endo- and plasma membranes

Correct determination of absolute values and polarities of liquid junction potentials (LJPs) is essential in patch clamp experiments for the estimation of ionic selectivities and activation voltages of ion channels. A simple approach for the correction of membrane voltages for LJPs has been develope...

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Bibliographic Details
Published inJournal of experimental botany Vol. 48; no. Special; pp. 361 - 364
Main Authors Amtmann, Anna, Sanders, Dale
Format Journal Article Conference Proceeding
LanguageEnglish
Published Oxford OXFORD UNIVERSITY PRESS 01.03.1997
Oxford University Press
Subjects
Amplifiers
Biological and medical sciences
Cell membranes
Cell physiology
Electric potential
Electrodes
Experimental procedures
Fundamental and applied biological sciences. Psychology
General Principles: Techniques and Models
Intracellular membranes
Ion channels
Liquids
Pipettes
Plant physiology and development
Plasma membrane and permeation
Vacuoles
Vegetals
Ionic selectivity
Plasma membrane
Patch clamp method
Procedure
Ionic channel
Membrane potential
Corrections
Liquid junction potential
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Summary:Correct determination of absolute values and polarities of liquid junction potentials (LJPs) is essential in patch clamp experiments for the estimation of ionic selectivities and activation voltages of ion channels. A simple approach for the correction of membrane voltages for LJPs has been developed. The method covers all combinations of LJPs between the solutions of bath, patch pipette and reference salt bridge and is applicable to all patch configurations on plasma membranes and endomembranes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/48.Special_Issue.361