Effects of arachidonic acid and cyclo (his-pro) on zinc transport across small intestine and muscle tissues

• Published in: Life sciences (1973) Vol. 70; no. 3; pp. 337 - 348
• Main Authors: Rosenthal, M.J., Hwang, I.K., Song, M.K.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 07.12.2001
• Subjects: Animals; Arachidonic acid; Arachidonic Acid - pharmacology; Biological Transport - drug effects; Cyclo (his-pro); Dose-Response Relationship, Drug; Intestine, Small - metabolism; Male; Muscle tissue; Muscles - metabolism; Peptides, Cyclic - pharmacology; Piperazines - pharmacology; Rat; Rats; Rats, Sprague-Dawley; Small intestine; Zinc; Zinc - metabolism; Cyclo (his-pro); Rat; Arachidonic acid; Muscle tissue; Small intestine; Zinc
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/S0024-3205(01)01395-9

Previously we have shown that arachidonic acid (AA) plus zinc or cyclo (his-pro) (CHP) plus zinc improve clinical signs of diabetes in streptozotocin-induced diabetic rats. Since streptozotocin destroys pancreatic β-cells, we hypothesize that the effect of either AA or CHP, plus zinc on glucose metabolism is via mobilization of intracellular zinc which in turn stimulates glucose uptake by peripheral tissues. We now report the relationship between zinc and AA and between zinc and CHP in controlling zinc influx and efflux across hindlimb muscle cells isolated from three-month old rats. Although CHP increased muscle zinc influx in a dose-dependent manner, AA was not effective. However, AA was more effective in stimulating zinc efflux than CHP. We have previously demonstrated that AA stimulates intestinal zinc uptake and absorption, and now present evidence that CHP also influences intestinal zinc transport. These results suggest that both AA and CHP affect glucose uptake in muscle cells via stimulating intestinal zinc absorption and muscle cell zinc flux.