Effect of the thin cell layer technique in the induction of somatic embryos in Pinus patula Schl. et Cham

• Published in: Journal of forestry research Vol. 30; no. 4; pp. 1535 - 1539
• Main Authors: Ramírez-Mosqueda, Marco A., Iglesias-Andreu, Lourdes G., Armas-Silva, Arturo A., Cruz-Gutiérrez, Esmeralda J., de la Torre-Sánchez, José F., Leyva-Ovalle, Otto R., Galán-Páez, Carlos M.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2019; Springer; Springer Nature B.V; Facultad de Ciencias Biológicas y Agropecuarias Región Córdoba-Orizaba, Universidad Veracruzana, C.P. 94945 Amatlan de los Reyes, Veracruz, Mexico%Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Av. de las Culturas Veracruzanas No. 101, Campus para la Cultura, las Artes y el Deporte, Col. Emiliano Zapata, C.P. 91090 Xalapa, Veracruz, Mexico%Centro Nacional de Recursos Genéticos-INIFAP, Boulevard de la Biodiversidad No. 400 Rancho las Cruces, C.P. 47600 Tepatitlén de Morelos, Jalisco, Mexico%Facultad de Ciencias Biológicas y Agropecuarias Región Córdoba-Orizaba, Universidad Veracruzana, C.P. 94945 Amatlan de los Reyes, Veracruz, Mexico; Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Av. de las Culturas Veracruzanas No. 101, Campus para la Cultura, las Artes y el Deporte, Col. Emiliano Zapata, C.P. 91090 Xalapa, Veracruz, Mexico
• Subjects: Biomedical and Life Sciences; Callus; Embryonic development; Embryonic growth stage; Embryos; Explants; Forestry; Germination; Life Sciences; Mass production; Methods; Micropropagation; Multiplication; Pine trees; Pinus patula; Production methods; Reforestation; Seedlings; Segments; Short Communication; Somatic embryogenesis; Species; Thin films; Thin cell layer; Micropropagation; Maturation; Somatic embryos; Pinus patula
• ISSN: 1007-662X; 1993-0607
• DOI: 10.1007/s11676-018-0663-0

Pinus patula is a species commonly used for reforestation in Mexico. However, efficient methods for the mass production seedlings are required. Micropropagation particularly by somatic embryogenesis provides an option for the rapid multiplication of high-quality, genetically improved material. This study induces somatic embryogenesis in this species using the thin cell layer (TCL) technique. Two sources of explants (complete immature embryos; lTCL segments from immature embryos) were evaluated. The efficiency of TCL from longitudinal sections [lTCL] and transverse [tTCL] was evaluated. The results show using thin cell layers from immature embryos cultivated in 16 light/8 dark hours achieves induction of somatic embryos. A higher percentage of embryogenic callus was obtained when tTCL segments were used as an explant source. These results produced somatic embryos from tTCL segments of an immature embryo without germinating the seed, making the process more time efficient. In addition, this technique can be used to generate somatic embryogenesis in forest species that have low germination rates.