Characterization of Histone H2A.X Expression in Testis and Specific Labeling of Germ Cells at the Commitment Stage of Meiosis with Histone H2A.X Promoter-Enhanced Green Fluorescent Protein Transgene
To study the complex molecular mechanisms of mammalian spermatogenesis, it would be useful to be able to isolate cells at each stage of differentiation, especially at the stage in which the cells switch from mitosis to meiosis. Currently, no useful marker proteins or gene promoters specific to this...
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Published in | Biology of reproduction Vol. 69; no. 4; pp. 1325 - 1329 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Madison, WI
Society for the Study of Reproduction
01.10.2003
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Subjects | |
Online Access | Get full text |
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Summary: | To study the complex molecular mechanisms of mammalian spermatogenesis, it would be useful to be able to isolate cells at
each stage of differentiation, especially at the stage in which the cells switch from mitosis to meiosis. Currently, no useful
marker proteins or gene promoters specific to this important stage are known. We report here a transgenic mouse line that
under the control of the promoter for a histone variant, H2A.X, expressed an enhanced green fluorescent protein (EGFP) in
cells at the stage of the mitosis-meiosis switch. Endogenous H2A.X is expressed in type A spermatogonia through meiotic prophase
spermatocytes in testis and in some somatic cells. However, despite the fact that its expression was driven by the H2A.X promoter,
the EGFP expressed in the transgenic mice specifically labeled only the intermediate spermatogonia stage through the meiotic
prophase spermatocyte stage in transgenic mice containing the â600-base pair H2A.X promoter/EGFP construct. Type A spermatogonia
and somatic cells of other organs were not labeled. This expression pattern made it possible to isolate living cells from
the testis of the transgenic mice at the stage of the mitosis-meiosis switch in spermatogenesis using EGFP fluorescence. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod.103.018952 |