Anti-inflammatory, analgesic and antioxidant activities of 3,4-oxo-isopropylidene-shikimic acid

• Published in: Pharmaceutical biology Vol. 54; no. 10; pp. 2282 - 2287
• Main Authors: Sun, Jin-Yao, You, Cui-Yu, Dong, Kai, You, Hai-Sheng, Xing, Jian-Feng
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.10.2016
• Subjects: Acetic Acid; Analgesics - pharmacology; Animals; Anti-Inflammatory Agents - pharmacology; Antioxidants - pharmacology; Carrageenan; Cotton Fiber; Dinoprostone - metabolism; Disease Models, Animal; Dose-Response Relationship, Drug; Edema - chemically induced; Edema - metabolism; Edema - prevention & control; Female; Granuloma, Foreign-Body - chemically induced; Granuloma, Foreign-Body - prevention & control; Hot Temperature; Hydroxyl Radical - chemistry; Illicium verum Hook.f; Indomethacin - pharmacology; Male; malondialdehyde; Malondialdehyde - metabolism; Mice, Inbred ICR; Pain - etiology; Pain - prevention & control; prostaglandin E2; Rats, Sprague-Dawley; Shikimic Acid - analogs & derivatives; Shikimic Acid - pharmacology; Superoxides - chemistry; Time Factors; xylene; Xylenes; xylene; Carrageenan; malondialdehyde; prostaglandin E2; Illicium verum Hook.f
• ISSN: 1388-0209; 1744-5116
• DOI: 10.3109/13880209.2016.1153663

Context 3,4-Oxo-isopropylidene-shikimic acid (ISA) is an analog of shikimic acid (SA). SA is extracted from the dry fruit of Illicium verum Hook. f. (Magnoliaceae), which has been used for treating stomachaches, skin inflammation and rheumatic pain. Objective To investigate the anti-inflammatory, analgesic and antioxidant activities of ISA. Materials and methods Analgesic and anti-inflammatory activities of ISA were evaluated using writhing, hot plate, xylene-induced ear oedema, carrageenan-induced paw oedema and cotton pellets-induced granuloma test, meanwhile the prostaglandin E 2 (PGE 2 ) and malondialdehyde (MDA) levels were assessed in the oedema paw tissue. ISA (60, 120 and 240 mg/kg in mice model and 50, 120 and 200 mg/kg in rat model) was administered orally, 30 min before induction of inflammation/pain. Additionally, ISA was administered for 12 d in rats from the day of cotton pellet implantation. The active oxygen species scavenging potencies of ISA (10 −3 -10 −5 M) were evaluated by the electron spin resonance spin-trapping technique. Results ISA caused a reduction of inflammation induced by xylene (18.1-31.4%), carrageenan (7.8-51.0%) and cotton pellets (11.4-24.0%). Furthermore, ISA decreased the production of PGE 2 and MDA in the rat paw tissue by 1.0-15.6% and 6.3-27.6%, respectively. ISA also reduced pain induced by acetic acid (15.6-48.9%) and hot plate (10.5-28.5%). Finally, ISA exhibited moderate antioxidant activity by scavenging the superoxide radical and hydroxyl radical with IC 50 values of 0.214 and 0.450 μg/mL, respectively. Discussion and conclusion Our findings confirmed the anti-inflammatory, analgesic and antioxidant activities of ISA.