Performance of the xTAG(R) Gastrointestinal Pathogen Panel, a Multiplex Molecular Assay for Simultaneous Detection of Bacterial, Viral, and Parasitic Causes of Infectious Gastroenteritis

• Published in: Journal of microbiology and biotechnology Vol. 23; no. 7; pp. 1041 - 1045
• Main Authors: Class, E.C., Leiden University Medical Center, Leiden, The Netherlands, Burnham, C.A.D., Washington University School of Medicine, St. Louis, USA, Mazzulli, T., University of Toronto, Toronto, Canada, Templeton, K., Royal Infirmary of Edinburgh, United Kingdom, Topin, F., Luminex B.V., Oosterhout, The Netherlands
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.07.2013; 한국미생물·생명공학회
• Subjects: Bacterial Infections - diagnosis; Biological and medical sciences; Biotechnology; Clinical Laboratory Techniques - methods; Feces - microbiology; Feces - parasitology; Feces - virology; Fundamental and applied biological sciences. Psychology; GASTROENTERITE; GASTROENTERITIS; Gastroenteritis - diagnosis; Humans; Luminex,multiplex,infectious diarrhea; Microbiology; Molecular Diagnostic Techniques - methods; Parasitic Diseases - diagnosis; Sensitivity and Specificity; Virus Diseases - diagnosis; 생물학; Gastroenteritis; Diarrhea; Parasite; Gastrointestinal; Luminex; infectious diarrhea; Infection; Virus; xTAG GPP; multiplex; Pathogenic; Bacteria; Detection
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1212.12042

The xTAGtex(R) Gastrointestinal Pathogen Panel (GPP) is a multiplexed molecular test for 15 gastrointestinal pathogens. The sensitivity and specificity of this test were assessed in 901 stool specimens collected from pediatric and adult patients at four clinical sites. A combination of conventional and molecular methods was used as comparator. Sensitivity could be determined for 12 of 15 pathogens and was 94.3% overall. The specificity across all 15 targets was 98.5%. Testing for the pathogen identified was not requested by the physician in 65% of specimens. The simultaneous detection of these 15 pathogens can provide physicians with a more comprehensive assessment of the etiology of diarrheal disease.