Construction of Antibody Phage Libraries and Their Application in Veterinary Immunovirology

Antibody phage display (APD) technology has revolutionized the field of immunovirology with its application in viral disease diagnostics and antiviral therapy. This robust and versatile technology allows the expression of an antibody fused to a phage coat protein on the surface of a filamentous phag...

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Bibliographic Details
Published inAntibodies (Basel) Vol. 9; no. 2; p. 21
Main Authors Bashir, Shahbaz, Paeshuyse, Jan
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 03.06.2020
MDPI
Subjects
Antibodies
Antigens
Antiviral agents
Bacteria
Binding sites
Cloning
Coat protein
Diagnostic systems
Economic importance
Genotypes
Health aspects
Immune system
Immunology
Immunoprophylaxis
immunovirology
Libraries
Library collections
Ligands
Monoclonal antibodies
monoclonal antibody
Nucleotide sequence
Peptides
Phage display
Phages
Phenotypes
Protein expression
Proteins
Review
Swine
Trends
Veterinary medicine
Viral antibodies
Viral diseases
Belgium
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Summary:Antibody phage display (APD) technology has revolutionized the field of immunovirology with its application in viral disease diagnostics and antiviral therapy. This robust and versatile technology allows the expression of an antibody fused to a phage coat protein on the surface of a filamentous phage. The DNA sequence coding for the antibody is packaged within the phage, linking the phenotype to genotype. Antibody phage display inherits the ability to rapidly generate and modify or improve high-affinity monoclonal antibodies, rendering it indispensable in immunology. In the last two decades, phage-display-derived antibodies have been extensively used in human medicine as diagnostic and therapeutic modalities. Recently, they are also gaining significant ground in veterinary medicine. Even though these advancements are mainly biased towards economically important animals such as chicken, cattle, and pigs, they are laying the foundation of fulfilling the unmet needs of veterinary medicine as antibody-based biologics in viral diagnostics, therapeutics, and immunoprophylaxis. This review provides a brief overview of the construction of antibody phage libraries and their application in diagnosis, prevention, and control of infectious viral diseases in veterinary medicine in detail.
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ISSN:2073-4468
2073-4468
DOI:10.3390/antib9020021