Nevskia aquatilis sp. nov. and Nevskia persephonica sp. nov., isolated from a mineral water aquifer and the emended description of the genus Nevskia

• Published in: Systematic and applied microbiology Vol. 35; no. 5; pp. 297 - 301
• Main Authors: Leandro, Tânia, França, Luís, Nobre, M. Fernanda, Schumann, Peter, Rosselló-Móra, Ramon, da Costa, Milton S.
• Format: Journal Article
• Language: English
• Published: München Elsevier GmbH 01.07.2012; Elsevier
• Subjects: Animal, plant and microbial ecology; Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Gammaproteobacteria - classification; Gammaproteobacteria - genetics; Gammaproteobacteria - isolation & purification; Gammaproteobacteria - physiology; Hydrogen-Ion Concentration; Microbial ecology; Microbiology; Molecular Sequence Data; Natural Springs - microbiology; Nevskia aquatilis; Nevskia persephonica; New taxa; Phylogeny; Portugal; Systematics; Temperature; Various environments (extraatmospheric space, air, water); Portugal; Europe; Nevskia persephonica; New taxa; Nevskia aquatilis; Natural mineral water; Description; Bottled water; Taxonomy; Bacteria; New species; Minerals; Aquifers
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/j.syapm.2012.05.001

Four isolates, with an optimum temperature of about 30°C and an optimum pH for growth of 6.0–6.5, were recovered from a borehole head of a mineral water aquifer in Portugal and from the stored bottles produced on site. Strains F2-63T and F2-178 were yellow-pigmented and formed non-motile rod-shaped cells. Strains G6M-30T and G6-54 were whitish-pigmented, translucent and form rod-shaped cells with a polar flagellum. The four strains were strictly aerobic, oxidase and catalase positive. The major fatty acids of strains F2-63T and F2-178 were C18:1ω7c and C16:0, and the major fatty acids of strains G6M-30T and G6-54 were C18:1ω7c and C16:1ω7c. Ubiquinone 8 was the major respiratory quinone. Based on 16S rRNA gene sequence analysis, physiological and biochemical characteristics two new species of the genus Nevskia are described; Nevskia aquatilis represented by strains F2-63T (=LMG 26345 =CECT 7897) and F2-178 (=LMG 26344 =CECT 7898) and Nevskia persephonica represented by strains G6M-30T (=DSM 24987 =CECT 7975) and G6-54 (=DSM 25048 =CECT 7976).