Cry29A and Cry30A: Two Novel δ-endotoxins Isolated from Bacillus thuringiensis serovar medellin

• Published in: Systematic and applied microbiology Vol. 26; no. 4; pp. 502 - 504
• Main Authors: Juárez-Pérez, Victor, Porcar, Manuel, Orduz, Sergio, Delécluse, Armelle
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01.11.2003; Elsevier
• Subjects: Aedes aegypti; Amino Acid Sequence; Animals; Anopheles stephensi; Bacillus thuringiensis; Bacillus thuringiensis - genetics; Bacillus thuringiensis - physiology; Bacillus thuringiensis serovar medellin; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - isolation & purification; Bacterial Proteins - metabolism; Bacterial Proteins - toxicity; Bacterial Toxins - chemistry; Bacterial Toxins - genetics; Bacterial Toxins - isolation & purification; Bacterial Toxins - metabolism; Bacterial Toxins - toxicity; Biological and medical sciences; Blotting, Southern; Cry29A; Cry30A; Culex pipiens; Culicidae - drug effects; DNA, Bacterial - chemistry; DNA, Bacterial - isolation & purification; Electrophoresis, Polyacrylamide Gel; Endotoxins - chemistry; Endotoxins - genetics; Endotoxins - isolation & purification; Endotoxins - toxicity; Fundamental and applied biological sciences. Psychology; Hemolysin Proteins; Microbiology; Molecular Sequence Data; Pest Control, Biological; Polymerase Chain Reaction; Sequence Analysis, DNA; Cry30A; Cry29A; Aedes aegypti; Bacillus thuringiensis serovar medellin; Microbiology; Taxonomy; Insecta; Bacillus thuringiensis; Bacillaceae; Endotoxin; Toxin; Bacillales; Arthropoda; Culicidae; Bacteria; Invertebrata; Diptera
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1078/072320203770865783

Two novel crystal protein genes from a highly mosquitocidal Bacillus thuringiensis serovar medellin strain were cloned and sequenced. The corresponding proteins, Cry29A and Cry30A, were nontoxic when tested individually against the mosquito species bioassayed ( Aedes aegypti, Culex pipiens and Anopheles stephensi). However, Cry29A synergized the toxicity of Cry11Bb against Aedes aegypti by a four-fold factor.