IgG reactivity with a 100-kDa tissue and endothelial cell antigen identified as topoisomerase 1 distinguishes between limited and diffuse systemic sclerosis patients

• Published in: Clinical immunology (Orlando, Fla.) Vol. 111; no. 3; pp. 241 - 251
• Main Authors: Garcı́a de la Peña-Lefebvre, Paloma, Chanseaud, Youri, Tamby, Mathieu C, Reinbolt, Joseph, Batteux, Frédéric, Allanore, Yannick, Kahan, André, Meyer, Olivier, Benveniste, Olivier, Boyer, Olivier, Guillevin, Loı̈c, Boissier, Marie-Christophe, Mouthon, Luc
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.06.2004; Elsevier
• Subjects: Antibody; Autoantibodies - analysis; Biological and medical sciences; Blotting, Western; Centromere - immunology; DNA Topoisomerases, Type I - immunology; Electrophoresis, Polyacrylamide Gel; Endothelial cell; Endothelial Cells - immunology; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulin G - analysis; Immunoglobulin M - analysis; Male; Medical sciences; Middle Aged; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Scleroderma, Systemic - immunology; Systemic sclerosis; Antibody; Endothelial cell; Systemic sclerosis; Human; Immunopathology; Connective tissue disease; Skin disease; Enzyme; EC 5.99.1.2; DNA topoisomerase; Autoantibody; IgG; Autoimmune disease; Antigen; Isomerases; Reactivity; Centromere; Systemic disease; Scleroderma
• ISSN: 1521-6616; 1521-7035
• DOI: 10.1016/j.clim.2004.01.005

We have analyzed antibody (Ab) reactivities of patients with limited systemic sclerosis (SSc) and anti-centromere Ab, patients with diffuse SSc and anti-topoisomerase 1 (anti-topo 1) Ab, patients with diffuse SSc without anti-topo 1 or anti-centromere Ab and age- and gender-matched healthy controls with normal human tissue and endothelial cell (EC) antigens. IgG reactivities with tissue antigens differed significantly between patients with anti-topo 1 Ab and patients with anti-centromere Ab. One 100-kDa band identified as topoisomerase 1 in macrovascular and microvascular EC extracts was recognized by IgG from patients with anti-topo 1 Ab and 50% of patients without specific Ab. IgG from patients with limited SSc and anti-centromere Ab, but not those of other patients or controls specifically recognized a 80-kDa band only in microvascular EC. Our results indicate that Ab from patients with limited or diffuse SSc with or without anti-topo 1 Ab exhibit specific and mutually exclusive reactivity patterns.