Low-Toxicity Self-Photosensitized Biohybrid Systems for Enhanced Light-Driven H2 Production

Nanoparticles (NPs) represent a potential optoelectronic source capable of significantly boosting hydrogen production; however, their inevitable cytotoxicity may lead to oxidative damage of bacterial cell membranes. In this study, we employed non-photosynthetic Escherichia coli K-12 as a model organ...

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Bibliographic Details
Published inInternational journal of molecular sciences Vol. 25; no. 6; p. 3085
Main Authors Wang, Yuelei, Liu, Yuqi, Bai, Long, Wang, Jueyu, Zhao, Na, Cui, Daizong, Zhao, Min
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 07.03.2024
MDPI
Subjects
Bacteria
biohybrid systems
Biosynthesis
CdS nanoparticles
Cytotoxicity
E coli
Environmental impact
Enzymes
Genes
Genetic engineering
Hydrogen
Hydrogen production
Light
Nanoparticles
Oxidative stress
peroxidase
Quantum dots
Radiation
Solar energy
Toxicity
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Summary:Nanoparticles (NPs) represent a potential optoelectronic source capable of significantly boosting hydrogen production; however, their inevitable cytotoxicity may lead to oxidative damage of bacterial cell membranes. In this study, we employed non-photosynthetic Escherichia coli K-12 as a model organism and utilized self-assembled cadmium sulfide (CdS) nanoparticles to construct a low-toxicity and hydrogen-production-enhancing self-photosensitive hybrid system. To mitigate the cytotoxicity of CdS NPs and synthesize biocompatible CdS NPs on the cell surface, we employed engineered E. coli (efeB/OE) for bioremediation, achieving this goal through the overexpression of the peroxidase enzyme (EfeB). A comparative analysis with E. coli–CdS revealed a significant downregulation of genes encoding oxidative stress proteins in efeB/OE–CdS post-irradiation. Atomic force microscopy (AFM) confirmed the stability of bacterial cell membranes. Due to the enhanced stability of the cell membrane, the hydrogen yield of the efeB/OE–CdS system increased by 1.3 times compared to the control, accompanied by a 49.1% reduction in malondialdehyde (MDA) content. This study proposes an effective strategy to alleviate the toxicity of mixed biological nanoparticle systems and efficiently harness optoelectronic electrons, thereby achieving higher hydrogen production in bioremediation.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25063085