Poly-glutamic dendrimer-based conjugates for cancer vaccination - a computational design for targeted delivery of antigens

• Published in: Journal of drug targeting Vol. 25; no. 9-10; pp. 873 - 880
• Main Authors: Moura, L. I. F., Martinho, N., Silva, L. C., Barata, T. S., Brocchini, S., Florindo, H. F., Zloh, M.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 26.11.2017
• Subjects: Amino Acid Sequence; Antigens, Neoplasm - administration & dosage; Antigens, Neoplasm - chemistry; Antigens, Neoplasm - metabolism; cancer; Cancer Vaccines - chemistry; Cancer Vaccines - genetics; Cancer Vaccines - metabolism; computational design; Computers, Molecular - trends; Dendrimers - administration & dosage; Dendrimers - chemistry; Dendrimers - metabolism; Drug Delivery Systems - methods; Drug Delivery Systems - trends; immunotherapy; Molecular Docking Simulation - trends; molecular dynamics simulation; Peptide dendrimer; poly(glutamic acid); Polyglutamic Acid - administration & dosage; Polyglutamic Acid - chemistry; Polyglutamic Acid - genetics; Polyglutamic Acid - metabolism; Protein Structure, Secondary; Protein Structure, Tertiary; receptor docking; tumour associated antigens; Vaccination - methods; Vaccination - trends; receptor docking; poly(glutamic acid); tumour associated antigens; immunotherapy; molecular dynamics simulation; cancer; computational design; Peptide dendrimer
• ISSN: 1061-186X; 1029-2330
• DOI: 10.1080/1061186X.2017.1363213

Computational techniques are useful to predict interaction models and molecular properties for the design of drug delivery systems, such as dendrimers. This work evaluated the impact of surface modifications of mannosamine-conjugated multifunctional poly(glutamic acid) (PG)-dendrimers as nanocarriers of the tumour associated antigens (TAA) MART-1, gp100:44 and gp100:209. Molecular dynamics simulations and docking studies were performed. Nitrobenzoxadiazole (NBD)-PG-G4-dendrimer displayed 64 carboxylic groups, however, the Frontier Molecular Orbital Theory study evidenced that only 32 of those were available to form covalent bonds. When the number of mannosamines conjugated to dendrimer was increased from 16 to 32, the dendrimer interacted with the receptor with higher affinity. However, 16 mannosamines-NBD-PG-G4-dendrimer was chosen to conjugate TAA for added functionality as no carboxylic end groups were available for further conjugation in the 32 mannosamines-dendrimer. Docking results showed that the majority of TAA-conjugated NBD-PG-G4-dendrimer demonstrated a favourable interaction with mannosamine binding site on mannose receptor, thus constituting a promising tool for TAA targeted delivery. Our in silico approach effectively narrows down the selection of the best candidates for the synthesis of functionalised PG-dendrimers with desired functionalities. These results will significantly reduce the time and efforts required to experimentally synthesise modified dendrimers for optimal antigen delivery.