Target deletion of the AAA ATPase PpCDC48II in Physcomitrella patens results in freezing sensitivity after cold acclimation
CDC48 is a highly conserved protein in eukaryotes and belongs to the AAA (ATPase associated with a variety of cellular activities) superfamily. It can interact with many different co-factors and form protein complexes that play important roles in various cellular processes. According to the Physcomi...
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Published in | Science China. Life sciences Vol. 55; no. 2; pp. 150 - 157 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Beijing
Science China Press
01.02.2012
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | CDC48 is a highly conserved protein in eukaryotes and belongs to the AAA (ATPase associated with a variety of cellular activities) superfamily. It can interact with many different co-factors and form protein complexes that play important roles in various cellular processes. According to the
Physcomitrella patens
database, one member of the ATPases, the cell cycle gene
PpCDC48II
, was cloned. PpCDC48II contains two typical ATPase modules and is highly homologous to AtCDC48A.
PpCDC48II
was up-regulated in mRNA levels after incubation at 0°C for 36 and 72 h. To further elucidate protein function, we disrupted the
PpCDC48II
gene by transforming
P. patens
with the corresponding linear genomic sequences. When treated to the same freezing stress, it was found that
PpCDC48II
knockout plants were less resistant to freezing treatment than wild type after acclimation. This suggested that
PpCDC48II
was an essential gene for low-temperature-induced freezing tolerance in
P. patens
cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1674-7305 1869-1889 |
DOI: | 10.1007/s11427-012-4277-z |