Protective effect of scutellarin on myocardial infarction induced by isoprenaline in rats

• Published in: Iranian journal of basic medical sciences Vol. 21; no. 3; pp. 267 - 276
• Main Authors: Huang, Haibo, Geng, Qianqian, Yao, Hong, Shen, Zhenhuang, Wu, Zhenhong, Miao, Xiaoqing, Shi, Peiying
• Format: Journal Article
• Language: English
• Published: Iran Mashhad University of Medical Sciences 01.03.2018
• Subjects: Apoptosis; Heart attacks; Inflammation; Isoprenaline; Myocardial infarction; Nitric oxide; Original; Oxidative stress; Rodents; Scutellarin; Myocardial infarction; Scutellarin; Oxidative stress; Isoprenaline; Inflammation; Apoptosis
• ISSN: 2008-3866; 2008-3874
• DOI: 10.22038/ijbms.2018.26110.6415

Scutellarin ( ) is the main effective constituent of which has anti-oxidant, anti-apoptotic, anti-inflammatory and other therapeutic properties. The purpose of this study was to investigate the protective effect of on myocardial infarction (MI) induced by isoprenaline (ISO). The rats were subcutaneously injected with ISO (45 mg/kg) on the first day, then single tail-intravenously injected with different doses of (10 mg/kg, 20 mg/kg, 40 mg/kg) for 7 consecutive days. The protective effect of on ISO-induced MI was evaluated by measuring markers of heart injury in serum, levels of lipid peroxidation, and antioxidants in heart tissue, observing pathological changes of tissue, and detecting quantified expression of apoptotic-related family members and inflammation. Compared with the model group, the concentration of troponin T (CTn-T) and troponin I (CTn-I), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) in the serum all decreased in the high dose group. The activities of superoxide dismutase (SOD), catalase (CAT), and the content of reduced glutathione (GSH) in heart increased, and the content of malondialdehyde (MDA) and inducible nitric oxide synthase (iNOS) decreased. In addition, the histopathologic aspects showed that pathological heart change was found in the model group, and was reduced to varying degrees in the group. Moreover, the expression of Bax, P53, Caspase3, Caspase9, cytochrome C, NGAL, NFκB, IL-1β and IL-6 in the heart decreased, while the expression of Bcl increased. could reduce the degree of MI induced by ISO by improving the antioxidant, anti-apoptotic, and anti-inflammatory capacities of the body.