Differential expression of BMP/SMAD signaling and ovarian-associated genes in the granulosa cells of FecB introgressed GMM sheep

• Published in: Systems biology in reproductive medicine Vol. 66; no. 3; pp. 185 - 201
• Main Authors: Kumar, Satish, Rajput, Pradeep Kumar, Bahire, Sangharatna V., Jyotsana, Basanti, Kumar, Vijay, Kumar, Davendra
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.05.2020
• Subjects: Animals; Bone Morphogenetic Protein Receptors, Type I - genetics; FecB; fecundity; Female; Fertility - genetics; gene expression; granulosa cells; Granulosa Cells - metabolism; Heterozygote; Mutation; Ovary - metabolism; Real-Time Polymerase Chain Reaction; Sheep; Sheep - genetics; Signal Transduction; Smad Proteins - metabolism; Transcriptome; Sheep; granulosa cells; gene expression; FecB; fecundity
• ISSN: 1939-6368; 1939-6376
• DOI: 10.1080/19396368.2019.1695977

The present study was conducted to analyze the mRNA expression of the BMP/SMAD signaling and steroidogenesis associated genes in the granulosa cells (GCs) of newly developed Booroola homozygous carrier GMM (FecB BB ) and non-carrier GMM (FecB ++ ) ewes through qRT-PCR. Results showed that the expression of BMP2 (P < 0.05) and BMP6 (P < 0.01) was significantly higher in the GCs of the homozygous carrier GMM (FecB BB ) than the non-carrier GMM (FecB ++ ) ewes, while the expression of BMP4 was significantly higher (P < 0.001) in the GCs of non-carrier GMM (FecB ++ ) than the homozygous carrier GMM (FecB BB ). In comparison, the expression of TGFβR1, BMPR1A, BMPR1B, and BMPRII was not significantly different between GCs of the homozygous carrier GMM (FecB BB ) and non-carrier GMM (FecB ++ ) ewes. Similarly, the expression of SMAD1, SMAD2, SMAD3, SMAD4, and SMAD5 was not significantly different between GCs of homozygous carrier GMM (FecB BB ) and non-carrier GMM (FecB ++ ). Further, expression of the INHIBIN, P4R, CYP11A1, and 3βHSD1 genes were not significantly different among the GCs of homozygous carrier GMM (FecB BB ) and non-carrier GMM (FecB ++ ), while the expression of StAR was significantly higher (P < 0.01) in the GCs of homozygous carrier GMM (FecB BB ) than that of GCs of non-carrier GMM (FecB ++ ) ewes. It is concluded that the FecB mutation significantly up-regulates the expression of BMP2, BMP6, and StAR genes and down-regulate the expression of BMP2 in granulosa cells of newly developed GMM ewes. BMP: Bone morphogenetic protein; TGFβ: Transforming growth factor-beta; SMAD: Fusion of Caenorhabditis elegans Sma genes and the Drosophila Mad, Mothers against decapentaplegic; GCs: Granulosa cells; GMM: Garole x Malpura x Malpura; FecB: Booroola fecundity; BMPR: Bone morphogenetic protein receptor; CYP11A1: Cytochrome P450 family 11 subfamily A member 1; StAR (Steroidogenic acute regulatory protein); 3βHSD1: 3 Beta-hydroxysteroid dehydrogenase; IGF: Insulin-like growth factor; ActR2: Activin receptor 2; ACVR1: Activin A receptor, type I; ACVR2: Activin A receptor, type II; ACVRL1: Activin A receptor like type 1; ACVR1B: Activin A receptor type 1B; ACVR1C: Activin A receptor type 1C; RFLP-PCR: Restriction fragment length polymorphism-polymerase chain reaction; qRT-PCR: Quantitative real-time PCR; ANOVA: Analysis of variance; P4R: Progesterone receptor: AMH: Anti mullerian hormone; RT-PCR: Reverse transcriptase-polymerase chain reaction; ER: Estrogen receptor; FSHR: Follicle stimulating hormone receptor.