A Survey on the Potential Mode of Inhibition for Oxalic Acid on Polyphenol Oxidase
The potential mode of inhibition for xalic acid on polyphenol oxidase (PPO) was investigated. The extent of inhibition was influenced not only by oxalic acid concentration but also by pH. Inhibition was most prominent at pH 4.0 where complete inhibition occurred at the 4‐mM oxalic acid concentration...
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Published in | Journal of food science Vol. 68; no. 8; pp. 2479 - 2485 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.10.2003
Institute of Food Technologists Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | The potential mode of inhibition for xalic acid on polyphenol oxidase (PPO) was investigated. The extent of inhibition was influenced not only by oxalic acid concentration but also by pH. Inhibition was most prominent at pH 4.0 where complete inhibition occurred at the 4‐mM oxalic acid concentration and was less evident at higher pH values. Inhibition of PPO by oxalic acid was due to its binding with copper to form an inactive complex, and the inhibition was characterized as noncompetitive. Oxalic acid diminished the catechol‐quinone product formation, and no quinone bleaching was observed. Oxalic acid was a more potent inhibitor of PPO compared with other structurally related acids. |
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Bibliography: | ArticleID:JFDS2479 ark:/67375/WNG-S9FV20N6-Z istex:D55F6DF59D4A42515D049A68D9B30EB2FBE43DF6 We are grateful to Seviye Yoruk, graduate student, Dept. of Industrial and Systems Engineering, Univ. of Florida, Gainesville, for her help in analyzing the data. Florida Agricultural Experiment Station Journal Series nr R‐09707. |
ISSN: | 0022-1147 1750-3841 |
DOI: | 10.1111/j.1365-2621.2003.tb07049.x |