Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion

• Published in: Biotechnology reports (Amsterdam, Netherlands) Vol. 28; p. e00526
• Main Authors: Ghio, Silvina, Bradanini, María B., Garrido, Mercedes M., Ontañon, Ornella M., Piccinni, Florencia E., Marrero Diaz de Villegas, Ruben, Talia, Paola M., Campos, Eleonora
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2020; Elsevier
• Subjects: Beta-glucan; Beta-glucosidase; Endoglucanse; GH1; GH8; Paenibacillus; Saccharification; GH1; Endoglucanse; Paenibacillus; Beta-glucan; GH8; Beta-glucosidase; Saccharification
• ISSN: 2215-017X; 2215-017X
• DOI: 10.1016/j.btre.2020.e00526

•Cel8Pa is an extracellular, halotolerant, broad substrate endoglucanase.•Bg1Pa is an intracellular β-glucosidase, with activity on cello oligosaccharides and high resistance to ethanol.•The concerted action of Cel8Pa and Bg1Pa has a synergistic effect on saccharification of β-glucans.•Cel8Pa and Bg1Pa are cold-stable and candidates for SSF ethanol 2 G processes. In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase (Cel8Pa) and a GH1 β-glucosidase (Bg1Pa) from Paenibacillus xylanivorans A59. Cel8Pa was active on a broad range of substrates, such as β-glucan from barley (24.5 IU/mg), lichenan (17.9 IU/mg), phosphoric acid swollen cellulose (PASC) (9.7 IU/mg), carboxi-methylcellulose (CMC) (7.3 IU/mg), chitosan (1.4 IU/mg) and xylan (0.4 IU/mg). Bg1Pa was active on cellobiose (C2) and cello-oligosaccharides up to C6, releasing glucose as the main product. When both enzymes were used jointly, there was a synergic effect in the conversion rate of polysaccharides to glucose. Cel8Pa and Bg1Pa presented important properties for simultaneous saccharification and fermentation (SSF) processes in second generation bioethanol production, such as tolerance to high concentration of glucose and ethanol.