Long‐chain Alkanes and ent‐Labdane‐type Diterpenes from Gymnosperma glutinosum with Cytotoxic Activity Against the Murine Lymphoma L5178Y‐R

• Published in: Phytotherapy research Vol. 26; no. 11; pp. 1632 - 1636
• Main Authors: Gomez‐Flores, R, Quintanilla‐Licea, R, Verde‐Star, M. J, Morado‐Castillo, R, Vázquez‐Díaz, D, Tamez‐Guerra, R, Tamez‐Guerra, P, Rodríguez‐Padilla, C
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.11.2012; Wiley
• Subjects: Alkanes - chemistry; Alkanes - isolation & purification; Alkanes - pharmacology; animal models; Animals; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; antitumor activity; Asteraceae - chemistry; Biological and medical sciences; Cell Line, Tumor; chloroform; cytotoxicity; Diterpenes - chemistry; Diterpenes - isolation & purification; Diterpenes - pharmacology; diterpenoids; fractionation; General pharmacology; Gymnosperma glutinosum; hexane; L5178Y-R; leaves; lymphoma; Lymphoma - pathology; lymphoma cells; Medical sciences; methanol; Mexico; Mice; murine; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; silica gel; Mexico; Antineoplastic agent; Terpenoid; Pharmacognosy; Rodentia; Cytotoxicity; Malignant hemopathy; Lymphoma; Medicinal plant; murine; lymphoma cells; Vertebrata; Mammalia; Mouse; antitumor activity; Lymphoproliferative syndrome; Animal; Gymnosperma glutinosum; Alkane; Plant origin; L5178Y-R; Diterpene; Labdane derivatives; Tumor cell; Cancer
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.4625

The antitumor potential of Gymnosperma glutinosum was previously reported using the in vitro and in vivo L5178Y‐R lymphoma murine model. The present study was carried out to isolate and identify the cytotoxic compounds present in the Gymnosperma glutinosum leaf hexane extract. Gymnosperma glutinosum was collected in the semi‐arid region of Escobedo, State of Nuevo León, México, but it is commonly found in northeastern Mexico; it is traditionally used as a treatment for diarrhea, ulcers and rheumatism. G. glutinosum leaves were extracted with hexane and further fractioned and subfractioned over silica gel by gradient elution with hexane, chloroform, ethyl acetate and methanol. The cytotoxicity of fractions and subfractions was assessed in vitro against L5178Y‐R lymphoma cells. Structure elucidation of the active compounds was determined by spectroscopic methods. Fractions and subfractions showed significant (p < 0.05) and concentration‐dependent 20% to 56% cytotoxicity against L5178Y‐R cells at concentrations ranging from 7.8 µg/mL to 500 µg/mL. The bioassay‐guided fractionation of the hexane extract resulted in the isolation and identification of the alkane hentriacontane and the diterpene ent‐labd‐7‐en‐13S,14R,15‐triol as the metabolites responsible for the activity.