Rate of non-adherent cell loss from long-term cultures of murine bone marrow: effect of medium conditioned by the adherent cell population

• Published in: Cell and tissue kinetics Vol. 18; no. 6; p. 613
• Main Authors: Janssen, W E, Snell, F M, Anbar, M
• Format: Journal Article
• Language: English
• Published: England 01.11.1985
• Subjects: Animals; Bone Marrow Cells; Cell Adhesion; Cell Survival; Cells, Cultured; Colony-Forming Units Assay; Culture Techniques - methods; Granulocytes - cytology; Hematopoietic Stem Cells - cytology; Macrophages - cytology; Mice; Mice, Inbred Strains; Spleen - cytology; Time Factors
• ISSN: 0008-8730
• DOI: 10.1111/j.1365-2184.1985.tb00704.x

The rate of random spontaneous cell loss from the non-adherent cell population of long-term cultures of murine bone marrow was determined. The measured rate of non-adherent cell loss is affected by previous conditioning of the medium in which the non-adherent cells are suspended. Specifically, the measured rate of non-adherent cell loss is significantly slowed when the non-adherent cells are suspended in medium conditioned in long-term haematopoietic cultures instead of fresh medium. It appears that a subset of the adherent cell population is the source of the factor or factors within the medium which result in this slower measured rate of non-adherent cell loss. This effect may be due to the stimulation to division of haematopoietic progenitor cells, offsetting the lysis of other non-adherent cells.