Effect of degree of unsaturation in dietary fatty acids on arachidonic acid mobilization by peritoneal macrophages

• Published in: Lipids Vol. 31; no. 6; pp. 661 - 666
• Main Authors: Mitjavila, M.T, Rodriguez, M.C, Saiz, M.P, Lloret, S, Moreno, J.J
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer‐Verlag 01.06.1996
• Subjects: Animals; Arachidonic Acid - chemistry; Arachidonic Acid - metabolism; Calcimycin - pharmacology; diet-related diseases; Docosahexaenoic Acids - metabolism; Eicosapentaenoic Acid - metabolism; Fatty Acids, Unsaturated - chemistry; Fatty Acids, Unsaturated - metabolism; Fish Oils - administration & dosage; human nutrition; Macrophage Activation; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - metabolism; Male; nutrition physiology; Opsonin Proteins; Rats; Rats, Sprague-Dawley; Structure-Activity Relationship; Tetradecanoylphorbol Acetate - pharmacology; Triglycerides - administration & dosage; Zymosan - pharmacology
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/bf02523839

Cells from rats fed with a tripalmitin diet showed a depletion of phospholipid arachidonate and n‐3 fatty acids such as eicosapentaenoic and docosahexaenoic acids (EPA and DHA). In rats fed fish oil diet, a significant reduction in archidonic acid (AA) content was observed whereas EPA and DHA were incorporated into membranes lipids. These changes in lipid composition of membranes did not affect cellular adherence, phagocytic capability, or [3H]AA incorporation. However, both tripalmitin and fish oil diets induced a decrease in [3H]AA mobilization stimulated by 4β‐phorbol‐12‐myristate 13‐acetate, A23187, or opsonized‐zymosan in rat peritoneal macrophages. These results demonstrate that the antiinflammatory effects of essential fatty acids deficiency or n‐3 enrichment diets may be associated with a decreased AA mobilization in resident rat peritoneal macrophages treated with proinflammatory agents.