Transepithelial Electrical Impedance Increase Following Porous Substrate Electroporation Enables Label‐Free Delivery
Porous substrate electroporation (PSEP) is a promising new method for intracellular delivery, yet fundamentals of PSEP are not well understood, especially the intermediate processes leading to delivery. PSEP is an electrical method, yet the relationship between PSEP and electrical impedance remains...
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Published in | Small (Weinheim an der Bergstrasse, Germany) Vol. 20; no. 25; pp. e2310221 - n/a |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
Wiley Subscription Services, Inc
01.06.2024
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Subjects | |
Online Access | Get full text |
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Summary: | Porous substrate electroporation (PSEP) is a promising new method for intracellular delivery, yet fundamentals of PSEP are not well understood, especially the intermediate processes leading to delivery. PSEP is an electrical method, yet the relationship between PSEP and electrical impedance remains underexplored. In this study, a device capable of measuring impedance and performing PSEP is developed and the changes in transepithelial electrical impedance (TEEI) are monitored. These measurements show TEEI increases following PSEP, unlike other electroporation methods. The authors then demonstrate how cell culture conditions and electrical waveforms influence this response. More importantly, TEEI response features are correlated with viability and delivery efficiency, allowing prediction of outcomes without fluorescent cargo, imaging, or image processing. This label‐free delivery also allows improved temporal resolution of transient processes following PSEP, which the authors expect will aid PSEP optimization for new cell types and cargos.
An integrated impedance measurement and porous substrate electroporation (PSEP) device is described. Impedance measurements from the device show transepithelial electrical impedance (TEEI) increases following PSEP, unlike other electroporation methods. A correlation between TEEI response and delivery outcomes is demonstrated, allowing assessment without fluorescent cargo, imaging, or image processing. This label‐free delivery also allows improved temporal resolution of processes following PSEP. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1613-6810 1613-6829 1613-6829 |
DOI: | 10.1002/smll.202310221 |