Polysaccharides from Pleurotus eryngii: Selective extraction methodologies and their modulatory effects on THP-1 macrophages

• Published in: Carbohydrate polymers Vol. 252; p. 117177
• Main Authors: Abreu, Hellen, Zavadinack, Matheus, Smiderle, Fhernanda R., Cipriani, Thales R., Cordeiro, Lucimara M.C., Iacomini, Marcello
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.01.2021
• Subjects: beta-Glucans - isolation & purification; beta-Glucans - pharmacology; Complex Mixtures - chemistry; Complex Mixtures - isolation & purification; Complex Mixtures - pharmacology; Fruiting Bodies, Fungal - chemistry; Fungal Polysaccharides - isolation & purification; Fungal Polysaccharides - pharmacology; Humans; Immunologic Factors - isolation & purification; Immunologic Factors - pharmacology; Immunomodulation; Immunostimulatory properties; Macrophages - drug effects; Mannogalactan; Molecular Structure; Pleurotus - chemistry; Pleurotus eryngii mushroom; Polysaccharides; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Structure-Activity Relationship; THP-1 Cells; β-D-glucansgg; Polysaccharides; Immunostimulatory properties; β-D-glucansgg; Pleurotus eryngii mushroom; Mannogalactan
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.117177

[Display omitted] •Pleurotus eryngii polysaccharides were selectively extracted and chemically characterized.•Mannogalactan was the main polysaccharide in cold-water extract (CW).•Linear (1→6)-β-d-glucan was the main polymer in hot-water extract (HW).•Autoclave-extracted (AE) fraction contained a mixture of at least four different α- and β-glucans.•CW and HW extracts were able to stimulate the release of NO, IL-1β and IL-10 by THP-1 macrophages. Polysaccharides from P. eryngii mushroom were selectively extracted using low-cost technologies (water at different conditions of temperature and pressure). Mannogalactan was the main polysaccharide in cold-water extracted fraction (CWEF), while a linear (1→6)-β-d-glucan was the main polymer in hot-water extracted fraction (HWEF). Autoclave-extracted fraction (AEF) contained a mixture of at least four different α- and β-glucans. The report of linear (1→6)-β-glucan and linear (1→3)-β-glucan is a new finding for P. eryngii fruiting bodies. The immunostimulatory properties of the fractions on THP-1 macrophages were studied. All fractions at 50, 250 and 500 μg/mL were not cytotoxic and produced different stimulus on NO, IL-1β and IL-10 secretion by the cells. Thus, our results showed that it is possible to concentrate different P. eryngii polysaccharides in selected fractions using a simple and low-cost procedure. Since biological effects depends on the polysaccharide structure, this technique allows the obtainment of fractions with distinct immunomodulatory activities.