Comparative analysis of cell wall surface glycan expression in Candida albicans and Saccharomyces cerevisiae yeasts by flow cytometry

• Published in: Journal of immunological methods Vol. 314; no. 1; pp. 90 - 102
• Main Authors: Martínez-Esparza, M., Sarazin, A., Jouy, N., Poulain, D., Jouault, T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 31.07.2006; Elsevier
• Subjects: Agglutination; Antibodies - immunology; Antibody Specificity; Antigens, Fungal - immunology; Biological and medical sciences; Candida albicans; Candida albicans - chemistry; Cell wall; Cell Wall - metabolism; Flow Cytometry - methods; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glycans; Isoantigens - immunology; Mannosides - immunology; Mannosides - metabolism; Microbiology; Molecular immunology; Mycological methods and techniques used in mycology; Mycology; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Surface markers; Techniques; Virulence factors; pAb; PLM; Candida albicans; Virulence factors; Glycans; Agglutination; Surface markers; Cell wall; mAb; Flow cytometry; Yeast; Virulence; Cell surface; Glycan; Immunological method; Fungi; Ascomycetes; Fungi Imperfecti; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/j.jim.2006.06.004

The yeast Candida albicans is an opportunistic pathogen, part of the normal human microbial flora that causes infections in immunocompromised individuals with a high morbidity and mortality levels. Recognition of yeasts by host cells is based on components of the yeast cell wall, which are considered part of its virulence attributes. Cell wall glycans play an important role in the continuous interchange that regulates the balance between saprophytism and parasitism, and also between resistance and infection. Some of these molecular entities are expressed both by the pathogenic yeast C. albicans and by Saccharomyces cerevisiae, a related non-pathogenic yeast, involving similar molecular mechanisms and receptors for recognition. In this work we have exploited flow cytometry methods for probing surface glycans of the yeasts. We compared glycan expression by C. albicans and by S. cerevisiae, and studied the effect of culture conditions. Our results show that the expression levels of α- and β-linked mannosides as well as β-glucans can be successfully evaluated by flow cytometry methods using different antibodies independent of agglutination reactions. We also found that the surface expression pattern of β-mannosides detected by monoclonal or polyclonal antibodies are differently modulated during the growth course. These data indicate that the yeast β-mannosides exposed on mannoproteins and/or phospholipomannan are increased in stationary phase, whereas those linked to mannan are not affected by the yeast growth phase. The cytometric method described here represents a useful tool to investigate to what extent C. albicans is able to regulate its glycan surface expression and therefore modify its virulence properties.