A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy
Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism b...
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| Published in | Gene Vol. 156; no. 2; pp. 223 - 227 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
24.04.1995
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| Subjects | |
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| Abstract | Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to
Meconopsis paniculata (Himalayan yellow poppy) DNA from a
Sau3A partial genomic plasmid library of
M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism between five species of
Meconopsis, M. aculeata, M. paniculata, M. simplicifolia, M. sinuata and
M. villosa, belonging to geographically isolated populations, was evident in genomic DNA filter hybridizations when probed with a clone (pIMS10) isolated from this library. Pooled DNA from seedlings originating from plants of individual populations of
M. paniculata, M. simplicifolia and
M. villosa gave similar band patterns, with respect to a given enzyme, suggesting intra-population genetic homogeneity |
|---|---|
| AbstractList | Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to
Meconopsis paniculata (Himalayan yellow poppy) DNA from a
Sau3A partial genomic plasmid library of
M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism between five species of
Meconopsis, M. aculeata, M. paniculata, M. simplicifolia, M. sinuata and
M. villosa, belonging to geographically isolated populations, was evident in genomic DNA filter hybridizations when probed with a clone (pIMS10) isolated from this library. Pooled DNA from seedlings originating from plants of individual populations of
M. paniculata, M. simplicifolia and
M. villosa gave similar band patterns, with respect to a given enzyme, suggesting intra-population genetic homogeneity Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism between five species of Meconopsis, M. aculeata, M. paniculata, M. simplicifolia, M. sinuata and M. villosa, belonging to geographically isolated populations, was evident in genomic DNA filter hybridizations when probed with a clone (pIMS10) isolated from this library. Pooled DNA from seedlings originating from plants of individual populations of M. paniculata, M. simplicifolia and M. villosa gave similar band patterns, with respect to a given enzyme, suggesting intra-population genetic homogeneity. |
| Author | Sulaiman, I.M. Ehtesham, N.Z. Hasnain, S.E. |
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| Cites_doi | 10.1007/BF00224242 10.1007/BF00225944 10.1111/j.1365-2052.1992.tb00226.x 10.1007/BF00042368 10.1111/j.1523-1739.1987.tb00053.x 10.7751/telopea19884809 10.1126/science.3029872 10.1016/0378-1119(92)90697-N 10.1098/rstb.1986.0004 10.1038/316076a0 10.2307/2444504 10.1016/0168-9452(91)90222-T 10.1002/bies.950020603 10.1016/0378-1119(94)00769-O |
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Newslett. contributor: fullname: Walbot – start-page: 312 year: 1991 ident: 10.1016/0378-1119(95)00087-M_BIB22 article-title: Oligonucleotide fingerprinting in plants and fungi contributor: fullname: Weising – volume: 153 start-page: 179 year: 1995 ident: 10.1016/0378-1119(95)00087-M_BIB4 article-title: Characterization of a DNA sequence that detects repetitive DNA elements in Asian rice gall midge (Orseolia oryzae: Wood-Mason) genome of potential use in DNA fingerprinting of biotypes publication-title: Gene doi: 10.1016/0378-1119(94)00769-O contributor: fullname: Ehtesham – volume: 21 start-page: 593 year: 1993 ident: 10.1016/0378-1119(95)00087-M_BIB16 article-title: Seed germination studies in three species of threatened, ornamental, Himalayan poppy, Meconopsis Vig. (Papaveraceae) publication-title: Seed Sci. Technol. contributor: fullname: Sulaiman |
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Meconopsis paniculata (Himalayan yellow poppy) DNA from... Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from... |
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| SubjectTerms | chemotaxonomy chi Cloning, Molecular Conservation of Natural Resources DNA fingerprinting DNA Probes DNA, Plant - genetics endangered species genetic homogeneity genetic polymorphism geographical variation geographically isolated populations Meconopsis meconopsis aculeata meconopsis paniculata meconopsis sinuata meconopsis villosa microsatellite Nucleic Acid Hybridization Phylogeny Plants - classification Plants - genetics Polymorphism, Restriction Fragment Length repetitive DNA repetitive sequences Repetitive Sequences, Nucleic Acid - genetics restriction mapping reverse genome hybridization satellite DNA |
| Title | A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy |
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