A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy

Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism b...

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Bibliographic Details
Published inGene Vol. 156; no. 2; pp. 223 - 227
Main Authors Sulaiman, I.M., Ehtesham, N.Z., Hasnain, S.E.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 24.04.1995
Subjects
chemotaxonomy
chi
Cloning, Molecular
Conservation of Natural Resources
DNA fingerprinting
DNA Probes
DNA, Plant - genetics
endangered species
genetic homogeneity
genetic polymorphism
geographical variation
geographically isolated populations
Meconopsis
meconopsis aculeata
meconopsis paniculata
meconopsis sinuata
meconopsis villosa
microsatellite
Nucleic Acid Hybridization
Phylogeny
Plants - classification
Plants - genetics
Polymorphism, Restriction Fragment Length
repetitive DNA
repetitive sequences
Repetitive Sequences, Nucleic Acid - genetics
restriction mapping
reverse genome hybridization
satellite DNA
genetic homogeneity
reverse genome hybridization
SDS
chi
nt
EtdBr
repetitive DNA
bp
Ap
LB
kb
microsatellite
oligo
DNA fingerprinting
geographically isolated populations
PCR
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Summary:Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism between five species of Meconopsis, M. aculeata, M. paniculata, M. simplicifolia, M. sinuata and M. villosa, belonging to geographically isolated populations, was evident in genomic DNA filter hybridizations when probed with a clone (pIMS10) isolated from this library. Pooled DNA from seedlings originating from plants of individual populations of M. paniculata, M. simplicifolia and M. villosa gave similar band patterns, with respect to a given enzyme, suggesting intra-population genetic homogeneity
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ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00087-M