An Injectable Nanocomposite Hydrogel for Potential Application of Vascularization and Tissue Repair

• Published in: Annals of biomedical engineering Vol. 48; no. 5; pp. 1511 - 1523
• Main Authors: Ding, Yilei, Zhao, An-sha, Liu, Tianmei, Wang, Ya-nan, Gao, Yuan, Li, Jing-an, Yang, Ping
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.05.2020; Springer Nature B.V
• Subjects: Angiogenesis; Animals; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Biophysics; Blood vessels; Cell adhesion & migration; Cell culture; Cell migration; Chemokine CXCL12 - administration & dosage; Chick Embryo; Chitosan; Chitosan - administration & dosage; Chorioallantoic membrane; Chorioallantoic Membrane - blood supply; Chorioallantoic Membrane - drug effects; Classical Mechanics; Coculture Techniques; Computer simulation; Embryos; Endothelial cells; Endothelial Cells - drug effects; Extracellular matrix; Gelatin; Gelatin - administration & dosage; Glycerophosphates - administration & dosage; Growth factors; Heart function; Hydrogels; Hydrogels - administration & dosage; Mesenchymal Stem Cells - drug effects; Mesenchyme; Muscles; Myocardial infarction; Myocardial Infarction - drug therapy; Myocytes, Smooth Muscle - drug effects; Nanocomposites; Nanocomposites - administration & dosage; Nanoparticles; Nanoparticles - administration & dosage; Neovascularization, Physiologic - drug effects; Oligopeptides - administration & dosage; Original Article; Peptides; Rats, Sprague-Dawley; Regeneration; Room temperature; SDF-1 protein; Smooth muscle; Stem cell transplantation; Stem cells; Tissue Engineering; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - administration & dosage; Vascularization; Nanoparticles; Injectable nanocomposite hydrogel; Tissue repair and regeneration; Vascularization
• ISSN: 0090-6964; 1573-9686; 1573-9686
• DOI: 10.1007/s10439-020-02471-7

In this contribution, an injectable hydrogel was developed with chitosan, gelatin, β-glycerphosphate and Arg-Gly-Asp (RGD) peptide: this hydrogel is liquid in room temperature and rapidly gels at 37 °C; RGD peptide promises better growth microenvironment for various cells, especially endothelial cells (EC), smooth muscle cells (SMC) and mesenchymal stem cells (MSC). Both stromal cell-derived factor-1 (SDF-1) nanoparticle and vascular endothelial growth factor (VEGF) nanoparticles were loaded in the injectable hydrogel to simulate the natural nanoparticles in the extracellular matrix (ECM) to promote angiogenesis. In vitro EC/SMC and MSC/SMC co-culture experiment indicated that the nanocomposite hydrogel accelerated constructing embryonic form of blood vessels, and chick embryo chorioallantoic membrane model demonstrated its ability of improving cells migration and blood vessel regeneration. We injected this nanocomposite hydrogel into rat myocardial infarction (MI) model and the results indicated that the rats heart function recovered better compared control group. We hope this injectable nanocomposite hydrogel may possess wider application in tissue engineering.